HR job title
- Professor and Associate Department Head
- Contact Info
- 1 979 845 0101
- Websites
Pellois, Jean-Philippe Professor
Positions
Our goal is to determine how proteins function in space and time in the context of complex cellular networks. We focus on chemistry-driven approaches to manipulate protein structure beyond what is feasible with standard genetics. In particular, we use semi-synthetic light-activatable proteins as biophysical probes to investigate protein mechanisms inside living cells. Areas of interest include the important but poorly understood process of protein S-acylation, signal transduction, and protein trafficking.
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- Works By Students
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Overview
Publications
selected publications
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academic article
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Arora, M., Ganugula, R., Kumar, N., Kaur, G., Pellois, J., Garg, P., & Kumar, M.
(2019).
Next-Generation Noncompetitive Nanosystems Based on Gambogic Acid: In silico Identification of Transferrin Receptor Binding Sites, Regulatory Shelf Stability, and Their Preliminary Safety in Healthy Rodents. ACS Applied Bio Materials.
2(8), 3540-3550.
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Pellois, J.
(2019).
Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays. BioEssays.
41(6), e1900031-1900031.
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Brock, D. J., Kondow-McConaghy, H. M., Hager, E. C., & Pellois, J.
(2019).
Endosomal Escape and Cytosolic Penetration of Macromolecules Mediated by Synthetic Delivery Agents. Bioconjugate Chemistry.
30(2), 293-304.
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Fang, Y. u., Lian, X., Huang, Y., Fu, G., Xiao, Z., Wang, Q. i., ... Zhou, H.
(2018).
Investigating Subcellular Compartment Targeting Effect of Porous Coordination Cages for Enhancing Cancer Nanotherapy. Small.
14(47), e1802709-1802709.
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Brock, D. J., Kustigian, L., Jiang, M., Graham, K., Wang, T., Erazo-Oliveras, A., ... Pellois, J.
(2018).
Efficient cell delivery mediated by lipid-specific endosomal escape of supercharged branched peptides. Traffic.
19(6), 421-435.
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Lian, X., Huang, Y., Zhu, Y., Fang, Y. u., Zhao, R., Joseph, E., ... Zhou, H.
(2018).
Enzyme-MOF Nanoreactor Activates Nontoxic Paracetamol for Cancer Therapy. Angewandte Chemie International Edition.
57(20), 5725-5730.
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Allen, J. K., Brock, D. J., Kondow-McConaghy, H. M., & Pellois, J.
(2018).
Efficient Delivery of Macromolecules into Human Cells by Improving the Endosomal Escape Activity of Cell-Penetrating Peptides: Lessons Learned from dfTAT and its Analogs. Biomolecules.
8(3), 50-50.
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Stubbendieck, R. M., Brock, D. J., Pellois, J., Gill, J. J., & Straight, P. D.
(2018).
Linearmycins are lytic membrane-targeting antibiotics. Journal of Antibiotics: an international journal devoted to research on bioactive microbial products.
71(3), 372-381.
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Najjar, K., Erazo-Oliveras, A., Mosior, J. W., Whitlock, M. J., Rostane, I., Cinclair, J. M., & Pellois, J.
(2017).
Unlocking Endosomal Entrapment with Supercharged Arginine-Rich Peptides. Bioconjugate Chemistry.
28(12), 2932-2941.
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Wang, T., Libardo, M., Angeles-Boza, A. M., & Pellois, J.
(2017).
Membrane Oxidation in Cell Delivery and Cell Killing Applications. ACS Chemical Biology.
12(5), 1170-1182.
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Najjar, K., Erazo-Oliveras, A., Brock, D. J., Wang, T., & Pellois, J.
(2017).
An l- to d-Amino Acid Conversion in an Endosomolytic Analog of the Cell-penetrating Peptide TAT Influences Proteolytic Stability, Endocytic Uptake, and Endosomal Escape. 292(3), 847-861.
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Lian, X., Erazo-Oliveras, A., Pellois, J., & Zhou, H.
(2017).
High efficiency and long-term intracellular activity of an enzymatic nanofactory based on metal-organic frameworks. Nature Communications.
8(1), 2075.
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Libardo, M., Wang, T., Pellois, J., & Angeles-Boza, A. M.
(2017).
How Does Membrane Oxidation Affect Cell Delivery and Cell Killing?. Trends in Biotechnology.
35(8), 686-690.
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Robison, A. D., Sun, S., Poyton, M. F., Johnson, G. A., Pellois, J., Jungwirth, P., Vazdar, M., & Cremer, P. S.
(2016).
Polyarginine Interacts More Strongly and Cooperatively than Polylysine with Phospholipid Bilayers. Journal of Physical Chemistry B.
120(35), 9287-9296.
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Wang, T., Sun, Y., Muthukrishnan, N., Erazo-Oliveras, A., Najjar, K., & Pellois, J.
(2016).
Membrane Oxidation Enables the Cytosolic Entry of Polyarginine Cell-penetrating Peptides. 291(15), 7902-7914.
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Erazo-Oliveras, A., Najjar, K., Truong, D., Wang, T., Brock, D. J., Prater, A. R., & Pellois, J.
(2016).
The Late Endosome and Its Lipid BMP Act as Gateways for Efficient Cytosolic Access of the Delivery Agent dfTAT and Its Macromolecular Cargos. Cell Chemical Biology.
23(5), 598-607.
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Najjar, K., Erazo-Oliveras, A., & Pellois, J.
(2015).
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT. Journal of Visualized Experiments.
2015(103),
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Patterson, J. L., Arenas-Gamboa, A. M., Wang, T., Hsiao, H., Howell, D. W., Pellois, J., Rice-Ficht, A., & Bondos, S. E.
(2015).
Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A.
103(4), 1546-1553.
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Li, Z., David, A., Albani, B. A., Pellois, J., Turro, C., & Dunbar, K. R.
(2014).
Optimizing the electronic properties of photoactive anticancer oxypyridine-bridged dirhodium(II,II) complexes. Journal of the American Chemical Society.
136(49), 17058-17070.
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Pena, B., David, A., Pavani, C., Baptista, M. S., Pellois, J., Turro, C., & Dunbar, K. R.
(2014).
Cytotoxicity Studies of Cyclometallated Ruthenium(II) Compounds: New Applications for Ruthenium Dyes. Organometallics.
33(5), 1100-1103.
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Johnson, G. A., Ellis, E. A., Kim, H., Muthukrishnan, N., Snavely, T., & Pellois, J.
(2014).
Photoinduced membrane damage of E. coli and S. aureus by the photosensitizer-antimicrobial peptide conjugate eosin-(KLAKLAK)2. PLoS ONE.
9(3), e91220-e91220.
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Meerovich, I., Muthukrishnan, N., Johnson, G. A., Erazo-Oliveras, A., & Pellois, J.
(2014).
Photodamage of lipid bilayers by irradiation of a fluorescently labeled cell-penetrating peptide. Biochimica et Biophysica Acta: international journal of biochemistry and biophysics.
1840(1), 507-515.
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Erazo-Oliveras, A., Najjar, K., Dayani, L., Wang, T., Johnson, G. A., & Pellois, J.
(2014).
Protein delivery into live cells by incubation with an endosomolytic agent. Nature Methods.
11(8), 861-867.
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Muthukrishnan, N., Donovan, S., & Pellois, J.
(2014).
The photolytic activity of poly-arginine cell penetrating peptides conjugated to carboxy-tetramethylrhodamine is modulated by arginine residue content and fluorophore conjugation site. Photochemistry and Photobiology.
90(5), 1034-1042.
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Muthukrishnan, N., Johnson, G. A., Erazo-Oliveras, A., & Pellois, J.
(2013).
Synergy between cell-penetrating peptides and singlet oxygen generators leads to efficient photolysis of membranes. 89(3), 625-630.
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Johnson, G. A., Muthukrishnan, N., & Pellois, J.
(2013).
Photoinactivation of Gram positive and Gram negative bacteria with the antimicrobial peptide (KLAKLAK)(2) conjugated to the hydrophilic photosensitizer eosin Y. Bioconjugate Chemistry.
24(1), 114-123.
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Erazo-Oliveras, A., Muthukrishnan, N., Baker, R., Wang, T., & Pellois, J.
(2012).
Improving the endosomal escape of cell-penetrating peptides and their cargos: strategies and challenges. Pharmaceuticals.
5(11), 1177-1209.
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Muthukrishnan, N., Johnson, G. A., Lim, J., Simanek, E. E., & Pellois, J.
(2012).
TAT-mediated photochemical internalization results in cell killing by causing the release of calcium into the cytosol of cells. Biochimica et Biophysica Acta: international journal of biochemistry and biophysics.
1820(11), 1734-1743.
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Srinivasan, D., Muthukrishnan, N., Johnson, G. A., Erazo-Oliveras, A., Lim, J., Simanek, E. E., & Pellois, J.
(2011).
Conjugation to the cell-penetrating peptide TAT potentiates the photodynamic effect of carboxytetramethylrhodamine. PLoS ONE.
6(3), e17732-e17732.
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Lee, Y., Johnson, G., Peltier, G. C., & Pellois, J.
(2011).
A HA2-Fusion tag limits the endosomal release of its protein cargo despite causing endosomal lysis. Biochimica et Biophysica Acta: international journal of biochemistry and biophysics.
1810(8), 752-758.
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Angeles-Boza, A. M., Erazo-Oliveras, A., Lee, Y., & Pellois, J.
(2010).
Generation of endosomolytic reagents by branching of cell-penetrating peptides: tools for the delivery of bioactive compounds to live cells in cis or trans. Bioconjugate Chemistry.
21(12), 2164-2167.
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Lee, Y., Johnson, G., & Pellois, J.
(2010).
Modeling of the endosomolytic activity of HA2-TAT peptides with red blood cells and ghosts. Biochemistry.
49(36), 7854-7866.
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Lee, Y., Erazo-Oliveras, A., & Pellois, J.
(2010).
Delivery of macromolecules into live cells by simple co-incubation with a peptide. ChemBioChem.
11(3), 325-330.
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Lim, J., Pellois, J., & Simanek, E. E.
(2010).
A retro-inverso TAT-like peptide designed to deliver cysteamine to cells. Bioorganic and Medicinal Chemistry Letters.
20(21), 6321-6323.
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Aguirre, J. D., Angeles-Boza, A. M., Chouai, A., Turro, C., Pellois, J., & Dunbar, K. R.
(2009).
Anticancer activity of heteroleptic diimine complexes of dirhodium: a study of intercalating properties, hydrophobicity and in cellulo activity. Dalton Transactions.
0(48), 10806-10812.
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Aguirre, J. D., Angeles-Boza, A. M., Chouai, A., Pellois, J., Turro, C., & Dunbar, K. R.
(2009).
Live cell cytotoxicity studies: documentation of the interactions of antitumor active dirhodium compounds with nuclear DNA. Journal of the American Chemical Society.
131(32), 11353-11360.
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Loudet, A., Han, J., Barhoumi, R., Pellois, J., Burghardt, R. C., & Burgess, K.
(2008).
Non-covalent delivery of proteins into mammalian cells. Organic and Biomolecular Chemistry.
6(24), 4516-4522.
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Lee, Y., Datta, S., & Pellois, J.
(2008).
Real-time fluorescence detection of protein transduction into live cells. Journal of the American Chemical Society.
130(8), 2398-2399.
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Hahn, M. E., Pellois, J., Vila-Perell, M., & Muir, T. W.
(2007).
Tunable photoactivation of a post-translationally modified signaling protein and its unmodified counterpart in live cells. ChemBioChem.
8(17), 2100-2105.
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Chatterjee, C., McGinty, R. K., Pellois, J., & Muir, T. W.
(2007).
Auxiliary-mediated site-specific peptide ubiquitylation. 46(16), 2814-2818.
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Pellois, J., & Muir, T. W.
(2006).
Semisynthetic proteins in mechanistic studies: using chemistry to go where nature can't. Current Opinion in Chemical Biology.
10(5), 487-491.
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Pellois, J., & Muir, T. W.
(2005).
A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells. Angewandte Chemie International Edition.
44(35), 5713-5717.
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Pellois, J., Hahn, M. E., & Muir, T. W.
(2004).
Simultaneous triggering of protein activity and fluorescence. Journal of the American Chemical Society.
126(23), 7170-7171.
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Srivannavit, O., Gulari, M., Gulari, E., LeProust, E., Pellois, J. P., Gao, X., & Zhou, X.
(2004).
Design and fabrication of microwell array chips for a solution-based, photogenerated acid-catalyzed parallel oligonuclotide DNA synthesis. Sensors and Actuators A: Physical.
116(1), 150-160.
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Gao, X., Pellois, J. P., Na, Y., Kim, Y., Gulari, E., & Zhou, X.
(2004).
High density peptide microarrays. In situ synthesis and applications. Molecular Diversity.
8(3), 177-187.
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Pellois, J. P., Zhou, X., Srivannavit, O., Zhou, T., Gulari, E., & Gao, X.
(2002).
Individually addressable parallel peptide synthesis on microchips. Nature Biotechnology.
20(9), 922-926.
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LeProust, E., Pellois, J. P., Yu, P., Zhang, H., Gao, X., Srivannavit, O., Gulari, E., & Zhou, X.
(2000).
Digital light-directed synthesis. A microarray platform that permits rapid reaction optimization on a combinatorial basis. ACS Combinatorial Science.
2(4), 349-354.
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Pellois, J. P., Wang, W., & Gao, X.
(2000).
Peptide synthesis based on t-Boc chemistry and solution photogenerated acids. 2(4), 355-360.
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Gao, X., Yu, P., LeProust, E., Sonigo, L., Pellois, J. P., & Zhang, H.
(1998).
Oligonucleotide Synthesis Using Solution Photogenerated Acids. Journal of the American Chemical Society.
120(48), 12698-12699.
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Arora, M., Ganugula, R., Kumar, N., Kaur, G., Pellois, J., Garg, P., & Kumar, M.
(2019).
Next-Generation Noncompetitive Nanosystems Based on Gambogic Acid: In silico Identification of Transferrin Receptor Binding Sites, Regulatory Shelf Stability, and Their Preliminary Safety in Healthy Rodents. ACS Applied Bio Materials.
2(8), 3540-3550.
Research
principal investigator on
- DEVELOPMENT OF CELLULAR DELIVERY TOOLS FOR BIOTECHNOLOGICAL AND THERAPEUTIC APPLICATIONS IN ANIMALS AND PLANTS awarded by National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States) 2019 - 2024
- Mechanisms and optimization of endosomal escape for delivery applications awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2015 - 2023
- I-Corps: Cell Delivery Agent awarded by National Science Foundation - (Arlington, Virginia, United States) 2017 - 2018
- Quantitative mapping of intracellular protein-protein interactomes in healthy and cancerous cells awarded by Cancer Prevention and Research Institute of Texas - (Austin, Texas, United States) 2016
- Delivery of Protein Biosensors Across the Plasma Membrane of Live Cells awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2009 - 2015
co-principal investigator on
investigator on
Teaching
teaching activities
- BICH440 Biochemistry I Instructor
- BICH491 Hnr-research Instructor
- BICH667 Molecular Probes Instructor
- BICH685 Directed Studies Instructor
- BICH689 Sptp: Info. Lit. In Biochem Instructor
- BICH689 Sptp: Membrane Bio & Biochem Instructor
- BICH689 Sptp: Oral Comm Instructor
- BICH689 Sptp: Oral Commun Instructor
- BICH689 Sptp:oral Commun Instructor
- BICH690 Theory Of Biochem Res Instructor
- BICH691 Research Instructor
- BICH697 Teaching Biochem Labs Instructor
- CHEM491 Hnr-research Instructor
- CHEM691 Research Instructor
- GENE491 Hnr-research Instructor
Works By Students
chaired theses and dissertations
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Erazo, Alfredo (2014-12). Cytosolic Delivery of Proteins, Peptides and Cell-Impermeable Small Molecules Into Live Cells Utilizing Virus-Inspired Multivalent Cell-Penetrating Peptides: Principles and Mechanisms.
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Johnson, Gregory Andrew (2013-07). Targeted Killing of Bacteria by Conjugation of a Soluble Photosensitizer to an Antimicrobial Peptide: Priniciples and Mechanisms.
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Lee, Ya-Jung (2012-07). Delivery of proteins in live cells with viral peptides: principles and mechanisms.
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Muthukrishnan, Nandhini (2014-05). Intracellular Delivery Using Fluorophore-CPP Conjugates and Light: Mechanisms and Implications.
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Najjar, Kristina (2017-07). Identifying and Characterizing Molecular Parameters that Modulate the Endosomal Escape of Cationic Cell- Penetrating Peptides: A Structure Activity Approach.
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Wang, Ting-Yi (2016-08). Membrane Oxidation Governed Direct Plasma Membrane Translocation of Cell-Penetrating Peptides in Live Cells: Mechanisms and Implications.
Background
education and training
Contact
first name
- Jean-Philippe
has last name
- Pellois
mailing address
-
Texas A&M University
Biochemistry & Biophysics
2128 TAMU
College Station, TX 77843-2128
USA
Other
- pellois@tamu.edu