Digital light-directed synthesis. A microarray platform that permits rapid reaction optimization on a combinatorial basis. Academic Article uri icon


  • Solution reactions using photogenerated reagents (Gao, X.; Yu, P.; LeProust, E.; Sonigo, L.; Pellois, J. P.; Zhang, H. J. Am. Chem. Soc. 1998, 120, 12698) are a potentially powerful means for combinatorial parallel synthesis of addressable molecular microarrays. In this report, we demonstrate that this chemistry permits combinatorial screening of reaction conditions on a microarray platform. Using this method of optimization and our reaction apparatus, efficient photogenerated acids and reaction conditions suitable for removal of the acid labile protection group on 5'-O of nucleotides are identified in a short period of time. The chemistry platform demonstrated opens new avenues for rapid, simultaneous investigation of multiple reactions using different reagents and reaction parameters directly on a solid support (e.g., a glass plate). The combinatorial screening method described may be extended to include general organic reactions employing photogenerated and conventional reagents as well as a microarray reaction device. This should be especially valuable for efficient synthesis of addressable organic compound libraries.

published proceedings

  • J Comb Chem

altmetric score

  • 6

author list (cited authors)

  • LeProust, E., Pellois, J. P., Yu, P., Zhang, H., Gao, X., Srivannavit, O., Gulari, E., & Zhou, X.

citation count

  • 68

complete list of authors

  • LeProust, E||Pellois, JP||Yu, P||Zhang, H||Gao, X||Srivannavit, O||Gulari, E||Zhou, X

publication date

  • July 2000