Digital light-directed synthesis. A microarray platform that permits rapid reaction optimization on a combinatorial basis.
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Solution reactions using photogenerated reagents (Gao, X.; Yu, P.; LeProust, E.; Sonigo, L.; Pellois, J. P.; Zhang, H. J. Am. Chem. Soc. 1998, 120, 12698) are a potentially powerful means for combinatorial parallel synthesis of addressable molecular microarrays. In this report, we demonstrate that this chemistry permits combinatorial screening of reaction conditions on a microarray platform. Using this method of optimization and our reaction apparatus, efficient photogenerated acids and reaction conditions suitable for removal of the acid labile protection group on 5'-O of nucleotides are identified in a short period of time. The chemistry platform demonstrated opens new avenues for rapid, simultaneous investigation of multiple reactions using different reagents and reaction parameters directly on a solid support (e.g., a glass plate). The combinatorial screening method described may be extended to include general organic reactions employing photogenerated and conventional reagents as well as a microarray reaction device. This should be especially valuable for efficient synthesis of addressable organic compound libraries.
author list (cited authors)
LeProust, E., Pellois, J. P., Yu, P., Zhang, H., Gao, X., Srivannavit, O., Gulari, E., & Zhou, X.
complete list of authors
LeProust, E||Pellois, JP||Yu, P||Zhang, H||Gao, X||Srivannavit, O||Gulari, E||Zhou, X