Protein delivery into live cells by incubation with an endosomolytic agent.
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We report that a tetramethylrhodamine-labeled dimer of the cell-penetrating peptide TAT, dfTAT, penetrates live cells by escaping from endosomes with high efficiency. By mediating endosomal leakage, dfTAT also delivers proteins into cultured cells after a simple co-incubation procedure. We achieved cytosolic delivery in several cell lines and primary cells and observed that only a relatively small amount of material remained trapped inside endosomes. Delivery did not require a binding interaction between dfTAT and a protein, multiple molecules could be delivered simultaneously, and delivery could be repeated. dfTAT-mediated delivery did not noticeably affect cell viability, cell proliferation or gene expression. dfTAT-based intracellular delivery should be useful for cell-based assays, cellular imaging applications and the ex vivo manipulation of cells.
author list (cited authors)
Erazo-Oliveras, A., Najjar, K., Dayani, L., Wang, T., Johnson, G. A., & Pellois, J.
complete list of authors
Erazo-Oliveras, Alfredo||Najjar, Kristina||Dayani, Laila||Wang, Ting-Yi||Johnson, Gregory A||Pellois, Jean-Philippe