Real-time fluorescence detection of protein transduction into live cells. Academic Article uri icon


  • We describe a protein probe with multiple fluorescence signals that can unambiguously detect protein translocation into live cells. When combined with fluorescence microscopy, this unique probe design allows for the first time the investigator to visualize and distinguish intact and degraded proteins with high spatial and temporal resolution. Thus, by using this approach, one can now compare the mechanisms and measure the efficiency of different delivery vectors, a prerequisite for the rational design of protein transduction systems with superior properties. Copyright 2008 American Chemical Society.

published proceedings

  • J Am Chem Soc

altmetric score

  • 7

author list (cited authors)

  • Lee, Y., Datta, S., & Pellois, J.

citation count

  • 46

complete list of authors

  • Lee, Ya-Jung||Datta, Silpi||Pellois, Jean-Philippe

publication date

  • February 2008