Real-time fluorescence detection of protein transduction into live cells. Academic Article

abstract

• We describe a protein probe with multiple fluorescence signals that can unambiguously detect protein translocation into live cells. When combined with fluorescence microscopy, this unique probe design allows for the first time the investigator to visualize and distinguish intact and degraded proteins with high spatial and temporal resolution. Thus, by using this approach, one can now compare the mechanisms and measure the efficiency of different delivery vectors, a prerequisite for the rational design of protein transduction systems with superior properties. Copyright 2008 American Chemical Society.

authors

• Pellois, Jean-Philippe

published proceedings

• J Am Chem Soc

altmetric score

• 7

author list (cited authors)

• Lee, Y., Datta, S., & Pellois, J.

citation count

• 46

complete list of authors

• Lee, Ya-Jung||Datta, Silpi||Pellois, Jean-Philippe

publication date

• February 2008

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Cytosol
• FLUORESCENCE
• Fluorescence Resonance Energy Transfer
• Fluorescent Dyes
• Hela Cells
• Humans
• Luminescent Proteins
• Molecular Structure
• Protein Transport

PubMed Central ID

• 18251482

Digital Object Identifier (DOI)

• 10.1021/ja7102026

start page

• 2398

end page

• 2399

volume

• 130

issue

• 8

URL

• http%3A%2F%2Fdx.doi.org%2F10.1021%2Fja7102026