HR job title
- Professor
- Contact Info
- 1 979 458 3375
- Websites
- ORCiD
- 0000-0003-0545-1508
Cruz-Reyes, Jorge Professor
Positions
We combine approaches in molecular genetics, structural biology, biochemistry, proteomics, and bioinformatics to study the amazing RNA biology of trypanosome parasites. One research line is on an RNA editing process by uridine insertion and deletion that creates amino acid coding triplets in most mRNAs. Yet a single error in the U-changes yields a frame-shift. Trypanosomes split from other eukaryotic lineages over a hundred million years ago, yet this editing has analogies with RNAi, CRISPR/Cas9, mRNA splicing and other systems directed by small non-coding RNAs (ncRNAs).
Research Areas
- Overview
- Publications
- Research
- Teaching
- Works By Students
- Background
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Overview
Publications
selected publications
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academic article
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Meehan, J., McDermott, S. M., Ivens, A., Goodall, Z., Chen, Z., Yu, Z., ... Cruz-Reyes, J.
(2023).
Trypanosome RNA helicase KREH2 differentially controls non-canonical editing and putative repressive structure via a novel proposed 'bifunctional' gRNA in mRNA A6. Nucleic Acids Research (NAR).
gkad453.
-
Aphasizheva, I., Alfonzo, J., Carnes, J., Cestari, I., Cruz-Reyes, J., Gringer, H. U., ... Aphasizhev, R.
(2020).
Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes. Trends in Parasitology.
36(4), 337-355.
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Chenna, B. C., Li, L., Mellott, D. M., Zhai, X., Siqueira-Neto, J. L., Calvet Alvarez, C., ... Meek, T. D.
(2020).
Peptidomimetic Vinyl Heterocyclic Inhibitors of Cruzain Effect Antitrypanosomal Activity. Journal of Medicinal Chemistry.
63(6), 3298-3316.
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Kumar, V., Doharey, P. K., Gulati, S., Meehan, J., Martinez, M. G., Hughes, K., Mooers, B., & Cruz-Reyes, J.
(2019).
Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes. 524702.
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Kumar, V., Doharey, P. K., Gulati, S., Meehan, J., Martinez, M. G., Hughes, K., Mooers, B., & Cruz-Reyes, J.
(2019).
Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes. PLoS ONE.
14(4), e0211525-e0211525.
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Cruz-Reyes, J., Mooers, B., Doharey, P. K., Meehan, J., & Gulati, S.
(2018).
Dynamic RNA holo-editosomes with subcomplex variants: Insights into the control of trypanosome editing. Wiley Interdisciplinary Reviews: RNA.
9(6), e1502.
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Kumar, V., Madina, B. R., Gulati, S., Vashisht, A. A., Kanyumbu, C., Pieters, B., ... Cruz-Reyes, J.
(2016).
REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. 291(11), 5753-5764.
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Cruz-Reyes, J., Mooers, B., Abu-Adas, Z., Kumar, V., & Gulati, S.
(2016).
DEAH-RHA helicaseZnf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes. http://www.smartscitech.com/index.php/RD.
3(2), e1336.
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Madina, B. R., Kumar, V., Mooers, B., & Cruz-Reyes, J.
(2015).
Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing. PLoS ONE.
10(4), e0123441-e0123441.
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Madina, B. R., Kumar, V., Metz, R., Mooers, B., Bundschuh, R., & Cruz-Reyes, J.
(2014).
Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition. RNA.
20(7), 1142-1152.
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Madina, B. R., Kuppan, G., Vashisht, A. A., Liang, Y., Downey, K. M., Wohlschlegel, J. A., ... Cruz-Reyes, J.
(2011).
Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei. RNA.
17(10), 1821-1830.
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Reifur, L., Yu, L. E., Cruz-Reyes, J., Vanhartesvelt, M., & Koslowsky, D. J.
(2010).
The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing. PLoS ONE.
5(8), e12235-e12235.
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Simpson, L., Aphasizhev, R., Lukes, J., & Cruz-Reyes, J.
(2010).
Guide to the nomenclature of kinetoplastid RNA editing: a proposal. Protist.
161(1), 2-6.
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Hernandez, A., Madina, B. R., Ro, K., Wohlschlegel, J. A., Willard, B., Kinter, M. T., & Cruz-Reyes, J.
(2010).
REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding. 285(2), 1220-1228.
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Alatortsev, V. S., Cruz-Reyes, J., Zhelonkina, A. G., & Sollner-Webb, B.
(2008).
Trypanosoma brucei RNA editing: coupled cycles of U deletion reveal processive activity of the editing complex. 28(7), 2437-2445.
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Hernandez, A., Panigrahi, A., Cifuentes-Rojas, C., Sacharidou, A., Stuart, K., & Cruz-Reyes, J.
(2008).
Determinants for association and guide RNA-directed endonuclease cleavage by purified RNA editing complexes from Trypanosoma brucei. Journal of Molecular Biology.
381(1), 35-48.
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Cifuentes-Rojas, C., Pavia, P., Hernandez, A., Osterwisch, D., Puerta, C., & Cruz-Reyes, J.
(2007).
Substrate determinants for RNA editing and editing complex interactions at a site for full-round U insertion. 282(7), 4265-4276.
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Miller, M. M., Halbig, K., Cruz-Reyes, J., & Read, L. K.
(2006).
RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reaction. RNA.
12(7), 1292-1303.
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Sacharidou, A., Cifuentes-Rojas, C., Halbig, K., Hernandez, A., Dangott, L. J., De Nova-Ocampo, M., & Cruz-Reyes, J.
(2006).
RNA editing complex interactions with a site for full-round U deletion in Trypanosoma brucei. RNA.
12(7), 1219-1228.
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Zhelonkina, A. G., O'Hearn, S. F., Law, J. A., Cruz-Reyes, J., Huang, C. E., Alatortsev, V. S., & Sollner-Webb, B.
(2006).
T. brucei RNA editing: action of the U-insertional TUTase within a U-deletion cycle. RNA.
12(3), 476-487.
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Halbig, K., Sacharidou, A., De Nova-Ocampo, M., & Cruz-Reyes, J.
(2006).
Preferential interaction of a 25kDa protein with an A6 pre-mRNA substrate for RNA editing in Trypanosoma brucei. INTERNATIONAL JOURNAL FOR PARASITOLOGY.
36(12), 1295-1304.
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Cifuentes-Rojas, C., Halbig, K., Sacharidou, A., De Nova-Ocampo, M., & Cruz-Reyes, J.
(2005).
Minimal pre-mRNA substrates with natural and converted sites for full-round U insertion and U deletion RNA editing in trypanosomes. Nucleic Acids Research (NAR).
33(20), 6610-6620.
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Halbig, K., De Nova-Ocampo, M., & Cruz-Reyes, J.
(2004).
Complete cycles of bloodstream trypanosome RNA editing in vitro. RNA.
10(6), 914-920.
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Cruz-Reyes, J., Zhelonkina, A. G., Huang, C. E., & Sollner-Webb, B.
(2002).
Distinct functions of two RNA ligases in active Trypanosoma brucei RNA editing complexes. Molecular and Cellular Biology.
22(13), 4652-4660.
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Huang, C. E., Cruz-Reyes, J., Zhelonkina, A. G., O'Hearn, S., Wirtz, E., & Sollner-Webb, B.
(2001).
Roles for ligases in the RNA editing complex of Trypanosoma brucei: band IV is needed for U-deletion and RNA repair. EMBO Journal.
20(17), 4694-4703.
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Cruz-Reyes, J., Zhelonkina, A., Rusche, L., & Sollner-Webb, B.
(2001).
Trypanosome RNA editing: simple guide RNA features enhance U deletion 100-fold. Molecular and Cellular Biology.
21(3), 884-892.
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Sollner-Webb, B., Cruz-Reyes, J., & Rusch, L. N.
(2001).
Direct sizing of RNA fragments using RNase-generated standards. METHODS IN ENZYMOLOGY.
342, 378-383.
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Sollner-Webb, B., Rusch, L. N., & Cruz-Reyes, J.
(2001).
Ribonuclease activities of trypanosome RNA editing complex directed to cleave specifically at a chosen site. METHODS IN ENZYMOLOGY.
341, 154-174.
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Cruz-Reyes, J., Rusch, L. N., & Sollner-Webb, B.
(1998).
Trypanosoma brucei U insertion and U deletion activities co-purify with an enzymatic editing complex but are differentially optimized. Nucleic Acids Research (NAR).
26(16), 3634-3639.
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Cruz-Reyes, J., Piller, K. J., Rusch, L. N., Mukherjee, M., & Sollner-Webb, B.
(1998).
Unexpected electrophoretic migration of RNA with different 3' termini causes a RNA sizing ambiguity that can be resolved using nuclease P1-generated sequencing ladders. Biochemistry.
37(17), 6059-6064.
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Cruz-Reyes, J., Rusch, L. N., Piller, K. J., & Sollner-Webb, B.
(1998).
T. brucei RNA editing: adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage. Molecular Cell.
1(3), 401-409.
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Rusch, L. N., Cruz-Reyes, J., Piller, K. J., & Sollner-Webb, B.
(1997).
Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria. EMBO Journal.
16(13), 4069-4081.
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Piller, K. J., Rusch, L. N., Cruz-Reyes, J., & Sollner-Webb, B.
(1997).
Resolution of the RNA editing gRNA-directed endonuclease from two other endonucleases of Trypanosoma brucei mitochondria. RNA.
3(3), 279-290.
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Cruz-Reyes, J., & Sollner-Webb, B.
(1996).
Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities. Proceedings of the National Academy of Sciences of the United States of America.
93(17), 8901-8906.
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Cruz-Reyes, J., ur-Rehman, T., Spice, W. M., & Ackers, J. P.
(1995).
A novel transcribed repeat element from Entamoeba histolytica. Gene.
166(1), 183-184.
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Cruz-Reyes, J., & Tata, J. R.
(1995).
Cloning, characterization and expression of two Xenopus bcl-2-like cell-survival genes. Gene.
158(2), 171-179.
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Spice, W. M., Cruz-Reyes, J. A., & Ackers, J. P.
(1993).
Molecular and cell biology of opportunistic infections in AIDS. Entamoeba histolytica. Molecular and cell biology of human diseases series.
2, 95-137.
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Cruz-Reyes, J. A., & Ackers, J. P.
(1992).
A DNA probe specific to pathogenic Entamoeba histolytica. Archives of Medical Research.
23(2), 271-275.
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Cruz-Reyes, J. A., Spice, W. M., Rehman, T., Gisborne, E., & Ackers, J. P.
(1992).
Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica. Parasitology (Cambridge).
104 ( Pt 2)(2), 239-246.
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Meehan, J., Ivens, A., Grote, S., Rodshagen, T., Chen, Z., Goode, C., ... Cruz-Reyes, J.
KREH2 helicase represses ND7 mRNA editing in procyclic-stage Trypanosoma brucei by opposite modulation of canonical and 'moonlighting' gRNA utilization creating a proposed mRNA structure. Nucleic Acids Research (NAR).
gkae699.
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Meehan, J., McDermott, S. M., Ivens, A., Goodall, Z., Chen, Z., Yu, Z., ... Cruz-Reyes, J.
(2023).
Trypanosome RNA helicase KREH2 differentially controls non-canonical editing and putative repressive structure via a novel proposed 'bifunctional' gRNA in mRNA A6. Nucleic Acids Research (NAR).
gkad453.
-
book
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Cruz-Reyes, J.
(2007).
RNA-protein interactions in assembled editing complexes in trypanosomes. Ed. 424.
Elsevier.
-
Cruz-Reyes, J.
(2007).
RNA-protein interactions in assembled editing complexes in trypanosomes. Ed. 424.
Elsevier.
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chapter
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CruzReyes, J., & Hernandez, A.
(2008).
ProteinProtein and RNAProtein Interactions in UInsertion/Deletion RNA Editing Complexes. RNA and DNA Editing.
(pp. 71-98).
Wiley.
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Spice, W. M., Cruz-Reyes, J. A., & Ackers, J. P.
(1992).
Entamoeba histolytica. Molecular and Cell Biology of Opportunistic Infections in AIDS.
(pp. 95-137).
Springer Nature.
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CruzReyes, J., & Hernandez, A.
(2008).
ProteinProtein and RNAProtein Interactions in UInsertion/Deletion RNA Editing Complexes. RNA and DNA Editing.
(pp. 71-98).
Wiley.
editor of
-
book
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Cruz-Reyes, J., & Gray, M. W.
(2018).
RNA Metabolism in Mitochondria
Cruz-Reyes, J., & Gray, M. W.. (Eds.),
Springer.
-
Cruz-Reyes, J., & Gray, M. W.
(2018).
RNA Metabolism in Mitochondria
Cruz-Reyes, J., & Gray, M. W.. (Eds.),
Springer.
Research
principal investigator on
- Protozoan Purine Phosphoribosyltransferases as Targets to Treat Malaria, African Trypanosomiasis and Chagas's Disease awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2017 - 2022
- Collaborative Research: RNA processing in trypanosomes mitochondria awarded by National Science Foundation - (Arlington, Virginia, United States) 2016 - 2020
- RNA Processing in Trypanosome Mitochondria awarded by National Science Foundation - (Arlington, Virginia, United States) 2011 - 2015
co-principal investigator on
investigator on
- Protozoan Purine Phosphoribosyltransferases as Targets to Treat Malaria, African Trypanosomiasis and Chagas's Disease awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2017 - 2022
- Protozoan Purine Phosphoribosyltransferases as Targets to Treat Malaria, African Trypanosomiasis and Chagas's Disease awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2017 - 2022
Teaching
teaching activities
- BICH431 Molecular Genetics Instructor
- BICH456 Rna World Instructor
- BICH489 Sptp: Rna Biology Instructor
- BICH491 Hnr-research Instructor
- BICH691 Research Instructor
- GENE431 Molecular Genetics Instructor
- GENE491 Hnr-research Instructor
- GENE691 Research Instructor
Works By Students
chaired theses and dissertations
-
Hernandez, Alfredo J. (2012-02). RNA Editing in Trypanosomes: Substrate Recognition and its Integration to RNA Metabolism.
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Kumar, Vikas (2016-04). The Function of a Central RNA Helicase RNP in Editosomes of Trypanosomes.
Background
education and training
Contact
first name
- Jorge
has last name
- Cruz-Reyes
mailing address
-
Texas A&M University
Biochemistry & Biophysics
2128 TAMU
College Station, TX 77843-2128
USA
Other
- cruzrey@tamu.edu