Unexpected electrophoretic migration of RNA with different 3' termini causes a RNA sizing ambiguity that can be resolved using nuclease P1-generated sequencing ladders. Academic Article uri icon


  • It has been widely believed that the electrophoretic migration difference of otherwise identical RNAs with a P versus OH terminus would be the same as occurs for DNA, a fairly reproducible approximately 1/2 nucleotide (nt) offset. RNA with a 5'-OH indeed migrates

published proceedings

  • Biochemistry

author list (cited authors)

  • Cruz-Reyes, J., Piller, K. J., Rusch, L. N., Mukherjee, M., & Sollner-Webb, B.

citation count

  • 31

complete list of authors

  • Cruz-Reyes, J||Piller, KJ||Rusché, LN||Mukherjee, M||Sollner-Webb, B

publication date

  • April 1998