REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding. Academic Article uri icon

abstract

  • Regulation of gene expression in kinetoplastid mitochondria is largely post-transcriptional and involves the orchestration of polycistronic RNA processing, 3'-terminal maturation, RNA editing, turnover, and translation; however, these processes remain poorly studied. Core editing complexes and their U-insertion/deletion activities are relatively well characterized, and a battery of ancillary factors has recently emerged. This study characterized a novel DExH-box RNA helicase, termed here REH2 (RNA editing associated helicase 2), in unique ribonucleoprotein complexes that exhibit unwinding and guide RNA binding activities, both of which required a double-stranded RNA-binding domain (dsRBD) and a functional helicase motif I of REH2. REH2 complexes and recently identified related particles share a multiprotein core but are distinguished by several differential polypeptides. Finally, REH2 associates transiently, via RNA, with editing complexes, mitochondrial ribosomes, and several ancillary factors that control editing and RNA stability. We propose that these putative higher order structures coordinate mitochondrial gene expression.

published proceedings

  • J Biol Chem

author list (cited authors)

  • Hernandez, A., Madina, B. R., Ro, K., Wohlschlegel, J. A., Willard, B., Kinter, M. T., & Cruz-Reyes, J.

citation count

  • 48

complete list of authors

  • Hernandez, Alfredo||Madina, Bhaskara Reddy||Ro, Kevin||Wohlschlegel, James A||Willard, Belinda||Kinter, Mike T||Cruz-Reyes, Jorge

publication date

  • January 1, 2010 11:11 AM