Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica. Academic Article

abstract

• Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet genotypically distinct. Another probe, R-2, while not distinguishing between the two forms of E. hystolytica, was able to differentiate between them and E. moshkovskii, which has morphologically identical cysts and trophozoites. A third probe, BR-1, identified strain-specific RFLPs.

authors

• Cruz-Reyes, Jorge

published proceedings

• Parasitology

author list (cited authors)

• Cruz-Reyes, J. A., Spice, W. M., Rehman, T., Gisborne, E., & Ackers, J. P.

citation count

• 29

complete list of authors

• Cruz-Reyes, JA||Spice, WM||Rehman, T||Gisborne, E||Ackers, JP

publication date

• April 1992

publisher

• Cambridge University Press (CUP)  Publisher

published in

• Parasitology (Cambridge)  Journal

keywords

• Animals
• Blotting, Northern
• Blotting, Southern
• DNA Fingerprinting
• DNA Probes
• DNA, Protozoan
• DNA, Recombinant
• DNA, Ribosomal
• Entamoeba Histolytica
• Humans
• Nucleic Acid Hybridization
• RNA, Protozoan
• RNA, Ribosomal
• Repetitive Sequences, Nucleic Acid
• Restriction Mapping

PubMed Central ID

• 1594290

Digital Object Identifier (DOI)

• 10.1017/s0031182000061679

start page

• 239

end page

• 246

volume

• 104 ( Pt 2)

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1017%2Fs0031182000061679