T. brucei RNA editing: adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage. Academic Article uri icon

abstract

  • In the currently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insertion and U-deletion cycles begin with a common kind of gRNA-directed cleavage. However, natural, altered, and mutationally interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated by ATP and ADP, while U-insertional cleavage shows completely reverse nucleotide effects. The adenosine nucleotides' effects appear to be allosteric and determined solely by sequences immediately adjacent to the anchor duplex. Both U-deletional and U-insertional cleavages are reasonably active at physiological mitochondrial ATP concentration. Notably, ATP and ADP markedly stimulate complete U-deletion and inhibit U-insertion reactions, reflecting their effects on cleavage. These plus previous results suggest that U deletion and U insertion are remarkably distinct.

published proceedings

  • Mol Cell

author list (cited authors)

  • Cruz-Reyes, J., Rusch, L. N., Piller, K. J., & Sollner-Webb, B.

citation count

  • 69

complete list of authors

  • Cruz-Reyes, J||Rusché, LN||Piller, KJ||Sollner-Webb, B

publication date

  • January 1, 1998 11:11 AM