HR job title
- Associate Professor
- Contact Info
Sczepanski, Jonathan Assistant Professor
Positions
- Assistant Professor, Chemistry, College of Arts and Sciences
Our primary research goals are to develop and apply novel tools for studying DNA damage in the context of chromatin and to explore new avenues for RNA-based therapeutics and diagnostics. By combining expertise in chemical biology, molecular biology, and molecular evolution, our lab addresses challenges associated with studying and targeting noncoding RNAs from a unique perspective. In addition, we utilize modern chemical biology techniques to develop designer chromatin systems for studying DNA damage. We are seeking motivated individuals who wish to gain experience in chemical biology, molecular biology, and in vitro evolution techniques.
- Overview
- Publications
- Research
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Overview
Publications
selected publications
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academic article
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Zhong, W., & Sczepanski, J. T.
(2023).
Chimeric d/l-DNA Probes of Base Excision Repair Enable Real-Time Monitoring of Thymine DNA Glycosylase Activity in Live Cells. Journal of the American Chemical Society.
145(31), 17066-17074.
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Yu, C., & Sczepanski, J. T.
(2023).
Correction: The influence of chirality on the behavior of oligonucleotides inside cells: revealing the potent cytotoxicity of G-rich l-RNA. Chemical Science.
14(26), 7393-7393.
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McGregor, L. A., Deckard, C. E., Smolen, J. A., Porter, G. M., & Sczepanski, J. T.
(2023).
Thymine DNA glycosylase mediates chromatin phase separation in a DNA methylationdependent manner. Journal of Biological Chemistry.
299(7), 104907-104907.
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McGregor, L. A., Zhu, B., Goetz, A. M., & Sczepanski, J. T.
(2023).
Thymine DNA glycosylase is an RNA-binding protein with high selectivity for G-rich sequences. Journal of Biological Chemistry.
299(4), 104590-104590.
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Aller Pellitero, M., Kundu, N., Sczepanski, J., & Arroyo-Currs, N.
(2023).
Os(II/III) complex supports pH-insensitive electrochemical DNA-based sensing with superior operational stability than the benchmark methylene blue reporter. The Analyst.
148(4), 806-813.
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Yu, C., & Sczepanski, J. T.
(2023).
The influence of chirality on the behavior of oligonucleotides inside cells: revealing the potent cytotoxicity of G-rich l-RNA. Chemical Science.
14(5), 1145-1154.
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Piwko, A. T., Han, X., Kabza, A. M., Dey, S., & Sczepanski, J. T.
(2022).
Inverse In Vitro Selection Enables Comprehensive Analysis of Cross-Chiral L-Aptamer Interactions. ChemBioChem.
23(24), e202200520.
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Clark, V., Waters, K., Orsburn, B., Bumpus, N. N., Kundu, N., Sczepanski, J. T., Ray, P., & Arroyo-Currs, N.
(2022).
Human Cyclophilin B Nuclease Activity Revealed via Nucleic Acid-Based Electrochemical Sensors. Angewandte Chemie International Edition.
61(45), e202211292.
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Shaver, A., Kundu, N., Young, B. E., Vieira, P. A., Sczepanski, J. T., & Arroyo-Currs, N.
(2022).
Correction to "Nuclease Hydrolysis Does Not Drive the Rapid Signaling Decay of DNA Aptamer-Based Electrochemical Sensors in Biological Fluids". Langmuir.
38(4), 1651-1651.
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Kabza, A. M., Kundu, N., Zhong, W., & Sczepanski, J. T.
(2022).
Integration of chemically modified nucleotides with DNA strand displacement reactions for applications in living systems. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology.
14(2), e1743.
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Li, J., & Sczepanski, J. T.
(2022).
Targeting a conserved structural element from the SARS-CoV-2 genome using l-DNA aptamers. RSC Chemical Biology.
3(1), 79-84.
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Shaver, A., Kundu, N., Young, B. E., Vieira, P. A., Sczepanski, J. T., & Arroyo-Currs, N.
(2021).
Nuclease Hydrolysis Does Not Drive the Rapid Signaling Decay of DNA Aptamer-Based Electrochemical Sensors in Biological Fluids. Langmuir.
37(17), 5213-5221.
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Yu, C., Kabza, A. M., & Sczepanski, J. T.
(2021).
Assembly of long L-RNA by native RNA ligation. Chemical Communications.
57(81), 10508-10511.
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Kundu, N., Young, B. E., & Sczepanski, J. T.
(2021).
Kinetics of heterochiral strand displacement from PNA-DNA heteroduplexes. Nucleic Acids Research (NAR).
49(11), 6114-6127.
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Deckard, C. E., & Sczepanski, J. T.
(2021).
Reversible chromatin condensation by the DNA repair and demethylation factor thymine DNA glycosylase. Nucleic Acids Research (NAR).
49(5), 2450-2459.
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Zhong, W., & Sczepanski, J. T.
(2020).
Direct Comparison of d‑DNA and l‑DNA Strand-Displacement Reactions in Living Mammalian Cells. ACS Synthetic Biology.
10(1), 209-212.
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Tjhung, K. F., Sczepanski, J. T., Murtfeldt, E. R., & Joyce, G. F.
(2020).
RNA-Catalyzed Cross-Chiral Polymerization of RNA. Journal of the American Chemical Society.
142(36), 15331-15339.
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Kabza, A. M., & Sczepanski, J. T.
(2020).
l-DNA-Based Catalytic Hairpin Assembly Circuit. Molecules.
25(4), 947-947.
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Dey, S., & Sczepanski, J. T.
(2020).
In vitro selection of l-DNA aptamers that bind a structured d-RNA molecule. Nucleic Acids Research (NAR).
48(4), 1669-1680.
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Kabza, A. M., Young, B. E., Kundu, N., & Sczepanski, J. T.
(2019).
Heterochiral Nucleic Acid Circuits. Emerging Topics in Life Sciences.
3(5), 501-506.
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Young, B. E., Kundu, N., & Sczepanski, J. T.
(2019).
Frontispiece: MirrorImage Oligonucleotides: History and Emerging Applications. Chemistry - A European Journal.
25(34),
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Young, B. E., Kundu, N., & Sczepanski, J. T.
(2019).
Mirror‐Image Oligonucleotides: History and Emerging Applications. Chemistry - A European Journal.
25(34), 7981-7990.
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Zhong, W., & Sczepanski, J. T.
(2019).
A Mirror Image Fluorogenic Aptamer Sensor for Live-Cell Imaging of MicroRNAs. ACS Sensors.
4(3), 566-570.
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Banerjee, D. R., Deckard, C. E., Zeng, Y. u., & Sczepanski, J. T.
(2019).
Acetylation of the histone H3 tail domain regulates base excision repair on higher-order chromatin structures. Scientific Reports.
9(1), 15972.
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Deckard, C. E., Banerjee, D. R., & Sczepanski, J. T.
(2019).
Chromatin Structure and the Pioneering Transcription Factor FOXA1 Regulate TDG-Mediated Removal of 5‑Formylcytosine from DNA. Journal of the American Chemical Society.
141(36), 14110-14114.
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Young, B. E., & Sczepanski, J. T.
(2019).
Heterochiral DNA Strand-Displacement Based on Chimeric d/l-Oligonucleotides. ACS Synthetic Biology.
8(12), 2756-2759.
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Deckard, C. E., & Sczepanski, J. T.
(2018).
Polycomb repressive complex 2 binds RNA irrespective of stereochemistry. Chemical Communications.
54(85), 12061-12064.
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Banerjee, D. R., Deckard, C. E., Elinski, M. B., Buzbee, M. L., Wang, W. W., Batteas, J. D., & Sczepanski, J. T.
(2018).
Plug-and-Play Approach for Preparing Chromatin Containing Site-Specific DNA Modifications: The Influence of Chromatin Structure on Base Excision Repair. Journal of the American Chemical Society.
140(26), 8260-8267.
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Kabza, A. M., & Sczepanski, J. T.
(2017).
Cover Feature: An lRNA Aptamer with Expanded Chemical Functionality that Inhibits MicroRNA Biogenesis (ChemBioChem 18/2017). ChemBioChem.
18(18), 1772-1772.
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Kabza, A. M., & Sczepanski, J. T.
(2017).
An l‐RNA Aptamer with Expanded Chemical Functionality that Inhibits MicroRNA Biogenesis. ChemBioChem.
18(18), 1824-1827.
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Kabza, A. M., Young, B. E., & Sczepanski, J. T.
(2017).
Heterochiral DNA Strand-Displacement Circuits. Journal of the American Chemical Society.
139(49), 17715-17718.
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Sczepanski, J. T., & Joyce, G. F
(2015).
Specific Inhibition of MicroRNA Processing Using L-RNA Aptamers. Journal of the American Chemical Society.
137(51), 16032-16037.
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Sczepanski, J. T., & Joyce, G. F.
(2014).
A cross-chiral RNA polymerase ribozyme. Nature.
515(7527), 440-442.
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Sczepanski, J. T., & Joyce, G. F
(2013).
Binding of a structured D-RNA molecule by an L-RNA aptamer. Journal of the American Chemical Society.
135(36), 13290-13293.
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Zhou, C., Sczepanski, J. T., & Greenberg, M. M
(2013).
Histone modification via rapid cleavage of C4'-oxidized abasic sites in nucleosome core particles. Journal of the American Chemical Society.
135(14), 5274-5277.
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Sczepanski, J. T., Zhou, C., & Greenberg, M. M
(2013).
Nucleosome core particle-catalyzed strand scission at abasic sites. Biochemistry.
52(12), 2157-2164.
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Zhou, C., Sczepanski, J. T., & Greenberg, M. M.
(2012).
Mechanistic studies on histone catalyzed cleavage of apyrimidinic/apurinic sites in nucleosome core particles. Journal of the American Chemical Society.
134(40), 16734-16741.
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Sczepanski, J. T., & Joyce, G. F.
(2012).
Synthetic evolving systems that implement a user-specified genetic code of arbitrary design. Chemistry and Biology.
19(10), 1324-1332.
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Sczepanski, J. T., Hiemstra, C. N., & Greenberg, M. M.
(2011).
Probing DNA interstrand cross-link formation by an oxidized abasic site using nonnative nucleotides. Bioorganic and Medicinal Chemistry.
19(19), 5788-5793.
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Wong, R. S., Sczepanski, J. T., & Greenberg, M. M
(2010).
Excision of a lyase-resistant oxidized abasic lesion from DNA. CHEMICAL RESEARCH IN TOXICOLOGY.
23(4), 766-770.
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Sczepanski, J. T., Wong, R. S., McKnight, J. N., Bowman, G. D., & Greenberg, M. M.
(2010).
Rapid DNA-protein cross-linking and strand scission by an abasic site in a nucleosome core particle. Proceedings of the National Academy of Sciences of the United States of America.
107(52), 22475-22480.
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Sczepanski, J. T., Jacobs, A. C., Van Houten, B., & Greenberg, M. M
(2009).
Double-strand break formation during nucleotide excision repair of a DNA interstrand cross-link. Biochemistry.
48(32), 7565-7567.
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Newman, C. A., Resendiz, M., Sczepanski, J. T., & Greenberg, M. M
(2009).
Photochemical generation and reactivity of the 5,6-dihydrouridin-6-yl radical. Journal of Organic Chemistry.
74(18), 7007-7012.
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Sczepanski, J. T., Jacobs, A. C., Majumdar, A., & Greenberg, M. M
(2009).
Scope and mechanism of interstrand cross-link formation by the C4'-oxidized abasic site. Journal of the American Chemical Society.
131(31), 11132-11139.
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Sczepanski, J., Jacobs, A., Van Houten, B., & Greenberg, M. M
(2009).
TOXI 85-Scope and mechanism of formation of interstrand crosslinks that give rise to double strand breaks upon nucleotide excision repair. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
238.
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Sczepanski, J. T., Jacobs, A. C., & Greenberg, M. M
(2008).
Self-promoted DNA interstrand cross-link formation by an abasic site. Journal of the American Chemical Society.
130(30), 9646-9647.
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Zhong, W., & Sczepanski, J. T.
(2023).
Chimeric d/l-DNA Probes of Base Excision Repair Enable Real-Time Monitoring of Thymine DNA Glycosylase Activity in Live Cells. Journal of the American Chemical Society.
145(31), 17066-17074.
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conference paper
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Zhou, C., Sczepanski, J. T., & Greenberg, M. M
(2012).
Rapid DNA strand scission at a basic sites in nucleosome core particles. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
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Sczepanski, J. T., & Joyce, G. F
(2012).
Synthesis and evolution of genetically encoded cross-replicating ribozymes. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
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Sczepanski, J., Jacobs, A., Van Houten, B., & Greenberg, M. M
(2010).
Scope and Mechanism of Formation of Interstrand Cross-Links That Give Rise to Double Strand Breaks upon Nucleotide Excision Repair. CHEMICAL RESEARCH IN TOXICOLOGY.
284-284.
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Sczepanski, J., Jacobs, A., & Greenberg, M. M
ORGN 114-Interstrand cross-link (ICL) formation by the C4'-oxidized abasic site in DNA. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
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Zhou, C., Sczepanski, J. T., & Greenberg, M. M
(2012).
Rapid DNA strand scission at a basic sites in nucleosome core particles. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
Research
principal investigator on
- Mirror Image Aptamers: Next Generation RNA-Binding Reagents for Basic Research and Therapeutic Applications awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2017 - 2022
- CLAP-seq: An Aptamer-Based Platform for Transcriptome-Wide Mapping of RNA Modifications awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2019 - 2021
- Mirror image DNA circuitry for complex microRNA analysis in live cells awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2019 - 2021
Teaching
teaching activities
- BICH491 Hnr-research Instructor
- CHEM227 Organic Chemistry I Instructor
- CHEM491 Hnr-research Instructor
- CHEM681 Seminar Instructor
- CHEM689 Sptp: Nucleic Acids Chemistry Instructor
- CHEM689 Sptp:nucleic Acids Chem Instructor
- CHEM689 Sptp:nucleic Acids Chemistry Instructor
- CHEM690 Theory Of Chem Resrch Instructor
- CHEM691 Research Instructor
- GENE491 Hnr-research Instructor
Background
education and training
awards and honors
Contact
first name
- Jonathan
has last name
- Sczepanski
mailing address
-
Texas A&M University
Chemistry
3255 TAMU
College Station, TX 77843-3255
USA
Other
- sczepanski@tamu.edu