Assembly of long L-RNA by native RNA ligation. Academic Article uri icon


  • Due to their intrinsic nuclease resistance, L-oligonucleotides are being increasingly utilized in the development of molecular tools and sensors. Yet, it remains challenging to synthesize long L-oligonucleotides, potential limiting future applications. Herein, we report straightforward and versitile approach to assemble long L-RNAs from two or more shorter fragments using T4 RNA ligase 1. We show that this approach is compatible with the assembly of several classes of functional L-RNA, which we highlight by generating a 124 nt L-RNA biosensor that functions in serum.

published proceedings

  • Chem Commun (Camb)

altmetric score

  • 2.25

author list (cited authors)

  • Yu, C., Kabza, A. M., & Sczepanski, J. T.

citation count

  • 0

complete list of authors

  • Yu, Chen-Hsu||Kabza, Adam M||Sczepanski, Jonathan T

publication date

  • January 2021