Probing DNA interstrand cross-link formation by an oxidized abasic site using nonnative nucleotides. Academic Article uri icon

abstract

  • The C4'-oxidized abasic site (C4-AP) forms two types of interstrand cross-links with the adjacent nucleotides in DNA. Previous experiments revealed that dG does not react with the lesion and that formation of one type of cross-link is catalyzed by the opposing dA. iso-GuanosinedC and 2-aminopurinedT base pairs were used to determine why dG does not cross-link with C4-AP despite its well known reactivity with other bis-electrophiles. 7-Deaza-2'-deoxyadenosine was used to probe the role of the nucleotide opposite C4-AP in the catalysis of interstrand cross-link formation.

published proceedings

  • Bioorg Med Chem

author list (cited authors)

  • Sczepanski, J. T., Hiemstra, C. N., & Greenberg, M. M.

citation count

  • 15

complete list of authors

  • Sczepanski, Jonathan T||Hiemstra, Christine N||Greenberg, Marc M

publication date

  • January 1, 2009 11:11 AM