Probing DNA interstrand cross-link formation by an oxidized abasic site using nonnative nucleotides Academic Article uri icon

abstract

  • The C4'-oxidized abasic site (C4-AP) forms two types of interstrand cross-links with the adjacent nucleotides in DNA. Previous experiments revealed that dG does not react with the lesion and that formation of one type of cross-link is catalyzed by the opposing dA. iso-Guanosine¬∑dC and 2-aminopurine¬∑dT base pairs were used to determine why dG does not cross-link with C4-AP despite its well known reactivity with other bis-electrophiles. 7-Deaza-2'-deoxyadenosine was used to probe the role of the nucleotide opposite C4-AP in the catalysis of interstrand cross-link formation.

author list (cited authors)

  • Sczepanski, J. T., Hiemstra, C. N., & Greenberg, M. M.

citation count

  • 14

publication date

  • August 2011