Synthetic evolving systems that implement a user-specified genetic code of arbitrary design. Academic Article uri icon


  • A synthetic genetic system, based on cross-replicating RNA enzymes, provides a means to evaluate alternative genetic codes that relate heritable information to corresponding molecular function. A special implementation of encoded combinatorial chemistry was used to construct complex populations of cross-replicating RNA enzymes in accordance with a user-specified code that relates genotype and phenotype on a molecule-by-molecule basis. The replicating enzymes were made to undergo self-sustained Darwinian evolution, resulting in the emergence of the most advantageous variants. These included both highly active enzymes that sustained the population as a whole and poorly active enzymes that survived as parasites of the active molecules. This evolutionary outcome was a consequence of the information capacity and fidelity of the genetic code, suggesting how these parameters should be adjusted to implement codes tailored to particular applications.

published proceedings

  • Chem Biol

altmetric score

  • 1.5

author list (cited authors)

  • Sczepanski, J. T., & Joyce, G. F.

citation count

  • 16

complete list of authors

  • Sczepanski, Jonathan T||Joyce, Gerald F

publication date

  • January 2012