Specific Inhibition of MicroRNA Processing Using L-RNA Aptamers. Academic Article

abstract

• In vitro selection was used to obtain l-RNA aptamers that bind the distal stem-loop of various precursor microRNAs (pre-miRs). These l-aptamers, termed "aptamiRs", bind their corresponding pre-miR target through highly specific tertiary interactions rather than Watson-Crick pairing. Formation of a pre-miR-aptamiR complex inhibits Dicer-mediated processing of the pre-miR, which is required to form the mature functional microRNA. One of the aptamiRs, which was selected to bind oncogenic pre-miR-155, inhibits Dicer processing under simulated physiological conditions, with an IC50 of 87 nM. Given that l-RNAs are intrinsically resistant to nuclease degradation, these results suggest that aptamiRs might be pursued as a new class of miR inhibitors.

authors

• Sczepanski, Jonathan

altmetric score

• 1.75

author list (cited authors)

• Sczepanski, J. T., & Joyce, G. F

citation count

• 31

complete list of authors

• Sczepanski, Jonathan T||Joyce, Gerald F

publication date

• January 2015

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Aptamers, Nucleotide
• Base Sequence
• Humans
• MicroRNAs
• Molecular Sequence Data
• RNA Processing, Post-Transcriptional

PubMed Central ID

• 26652064

Digital Object Identifier (DOI)

• 10.1021/jacs.5b06696

start page

• 16032

end page

• 16037

volume

• 137

issue

• 51

URL

• http%3A%2F%2Fdx.doi.org%2F10.1021%2Fjacs.5b06696