Double-strand break formation during nucleotide excision repair of a DNA interstrand cross-link. Academic Article

abstract

• The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences the cleavage sites. Moreover, in approximately 15% of the molecules, the strand opposite the nick is incised, resulting in a more toxic double-strand break. This is the first example in which an interstrand cross-link is converted by nucleotide excision misrepair into a more deleterious double-strand break.

authors

• Sczepanski, Jonathan

author list (cited authors)

• Sczepanski, J. T., Jacobs, A. C., Van Houten, B., & Greenberg, M. M

citation count

• 44

complete list of authors

• Sczepanski, Jonathan T||Jacobs, Aaron C||Van Houten, Bennett||Greenberg, Marc M

publication date

• January 2009

publisher

• American Chemical Society (ACS)  Publisher

published in

• Biochemistry  Journal

keywords

• Base Sequence
• DNA
• DNA Repair
• Dna Breaks, Double-stranded
• Endodeoxyribonucleases
• Escherichia Coli Proteins
• Molecular Sequence Data
• Molecular Structure

Digital Object Identifier (DOI)

• 10.1021/bi901006b

start page

• 7565

end page

• 7567

volume

• 48

issue

• 32

URL

• http%3A%2F%2Fdx.doi.org%2F10.1021%2Fbi901006b