Double-Strand Break Formation during Nucleotide Excision Repair of a DNA Interstrand Cross-Link Academic Article uri icon

abstract

  • The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences the cleavage sites. Moreover, in approximately 15% of the molecules, the strand opposite the nick is incised, resulting in a more toxic double-strand break. This is the first example in which an interstrand cross-link is converted by nucleotide excision misrepair into a more deleterious double-strand break.

author list (cited authors)

  • Sczepanski, J. T., Jacobs, A. C., Van Houten, B., & Greenberg, M. M.

publication date

  • January 1, 2009 11:11 AM