HR job title
- Retired Professor
- Contact Info
- 1 979 862 1495
Scholthof, Herman Professor
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Overview
Publications
selected publications
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academic article
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DeMell, A., Alvarado, V., & Scholthof, H. B.
(2023).
Molecular perspectives on age-related resistance of plants to (viral) pathogens. New Phytologist.
-
Scholthof, H. B., & Scholthof, K.
(2023).
Plant virology: an RNA treasure trove. Trends in Plant Science.
S1360-1385(23)00223-6.
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Chiong, K. T., Cody, W. B., & Scholthof, H. B.
(2021).
RNA silencing suppressor-influenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing. Scientific Reports.
11(1), 6769.
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Cody, W. B., & Scholthof, H. B.
(2020).
Native Processing of Single Guide RNA Transcripts to Create Catalytic Cas9/Single Guide RNA Complexes in Planta. PLANT PHYSIOLOGY.
184(2), 1194-1206.
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Cody, W. B., & Scholthof, H. B.
(2019).
Plant Virus Vectors 3.0: Transitioning into Synthetic Genomics. Annual Review of Phytopathology.
57(1), 211-230.
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Zhumabek, A. T., Abeuova, L. S., Mukhametzhanov, N. S., Scholthof, H. B., Ramankulov, Y. M., & Manabayeva, S. A.
(2018).
Transient expression of a bovine leukemia virus envelope glycoprotein in plants by a recombinant TBSV vector. Journal of Virological Methods.
255, 1-7.
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Mendoza, M. R., Payne, A. N., Castillo, S., Crocker, M., Shaw, B. D., & Scholthof, H. B.
(2017).
Expression of Separate Proteins in the Same Plant Leaves and Cells Using Two Independent Virus-Based Gene Vectors. Frontiers in Plant Science.
8, 1808.
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Cody, W. B., Scholthof, H. B., & Mirkov, T. E.
(2017).
Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector. PLANT PHYSIOLOGY.
175(1), 23-35.
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Odokonyero, D., Mendoza, M. R., Moffett, P., & Scholthof, H. B.
(2017).
Tobacco rattle virus (TRV)-Mediated Silencing of Nicotiana benthamiana ARGONAUTES (NbAGOs) Reveals New Antiviral Candidates and Dominant Effects of TRV-NbAGO1. Phytopathology.
107(8), 977-987.
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Omarov, R. T., Ciomperlik, J., & Scholthof, H. B.
(2016).
An in vitro reprogrammable antiviral RISC with size-preferential ribonuclease activity. Virology.
490, 41-48.
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Odokonyero, D., Mendoza, M. R., Alvarado, V. Y., Zhang, J., Wang, X., & Scholthof, H. B.
(2015).
Transgenic down-regulation of ARGONAUTE2 expression in Nicotiana benthamiana interferes with several layers of antiviral defenses. Virology.
486, 209-218.
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Odokonyero, D., Mendoza, M. R., Alvarado, V. Y., Zhang, J., Wang, X., & Scholthof, H. B.
(2015).
Transgenic down-regulation of ARGONAUTE2 expression in Nicotiana benthamiana interferes with several layers of antiviral defenses. Virology.
486, 209-218.
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Shamekova, M., Mendoza, M. R., Hsieh, Y., Lindbo, J., Omarov, R. T., & Scholthof, H. B.
(2014).
Tombusvirus-based vector systems to permit over-expression of genes or that serve as sensors of antiviral RNA silencing in plants. Virology.
452-453, 159-165.
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Gao, S., Damaj, M. B., Park, J., Beyene, G., Buenrostro-Nava, M. T., Molina, J., ... Mirkov, T. E.
(2013).
Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors. PLoS ONE.
8(6), e66046-e66046.
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Manabayeva, S. A., Shamekova, M., Park, J., Ding, X. S., Nelson, R. S., Hsieh, Y., Omarov, R. T., & Scholthof, H. B.
(2013).
Differential requirements for Tombusvirus coat protein and P19 in plants following leaf versus root inoculation. Virology.
439(2), 89-96.
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Omarov, R. T., & Scholthof, H. B.
(2012).
Biological chemistry of virus-encoded suppressors of RNA silencing: an overview. 894, 39-56.
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Alvarado, V. Y., Odokonyero, D., Duncan, O., Mirkov, T. E., & Scholthof, H. B.
(2012).
Molecular and physiological properties associated with zebra complex disease in potatoes and its relation with Candidatus Liberibacter contents in psyllid vectors. PLoS ONE.
7(5), e37345-e37345.
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Seaberg, B. L., Hsieh, Y., Scholthof, K., & Scholthof, H. B.
(2012).
Host impact on the stability of a plant virus gene vector as measured by a new fluorescent local lesion passaging assay. Journal of Virological Methods.
179(2), 289-294.
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Omarov, R. T., & Scholthof, H. B.
(2012).
Biological chemistry of virus-encoded suppressors of RNA silencing: an overview. Methods in molecular biology (Clifton, N.J.).
894, 39-56.
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Saxena, P., Hsieh, Y., Alvarado, V. Y., Sainsbury, F., Saunders, K., Lomonossoff, G. P., & Scholthof, H. B.
(2011).
Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless. Plant Biotechnology Journal.
9(6), 703-712.
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Scholthof, H. B., Alvarado, V. Y., Vega-Arreguin, J. C., Ciomperlik, J., Odokonyero, D., Brosseau, C., ... Moffett, P.
(2011).
Identification of an ARGONAUTE for antiviral RNA silencing in Nicotiana benthamiana. 156(3), 1548-1555.
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Ciomperlik, J. J., Omarov, R. T., & Scholthof, H. B.
(2011).
An antiviral RISC isolated from Tobacco rattle virus-infected plants. Virology.
412(1), 117-124.
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Scholthof, K., & Scholthof, H. B.
(2011).
Induction and suppression of RNA silencing: insights from plant viral infections--a BARD workshop report. Plant Molecular Biology: An International Journal on Molecular Biology, Molecular Genetics and Biochemistry.
75(3), 205-210.
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Alvarado, V. Y., & Scholthof, H. B.
(2011).
AGO2: A New Argonaute Compromising Plant Virus Accumulation. Frontiers in Plant Science.
2(JAN), 112.
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Everett, A. L., Scholthof, H. B., & Scholthof, K.
(2010).
Satellite panicum mosaic virus coat protein enhances the performance of plant virus gene vectors. Virology.
396(1), 37-46.
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Alvarado, V., & Scholthof, H. B.
(2009).
Plant responses against invasive nucleic acids: RNA silencing and its suppression by plant viral pathogens. Seminars in Cell and Developmental Biology.
20(9), 1032-1040.
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Wen, A., Mallik, I., Alvarado, V. Y., Pasche, J. S., Wang, X., Li, W., ... Gudmestad, N. C.
(2009).
Detection, Distribution, and Genetic Variability of 'Candidatus Liberibacter' Species Associated with Zebra Complex Disease of Potato in North America. Plant Disease.
93(11), 1102-1115.
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Hsieh, Y., Omarov, R. T., & Scholthof, H. B.
(2009).
Diverse and newly recognized effects associated with short interfering RNA binding site modifications on the Tomato bushy stunt virus p19 silencing suppressor. Journal of Virology.
83(5), 2188-2200.
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Scholthof, H. B.
(2007).
Heterologous expression of viral RNA interference suppressors: RISC management. PLANT PHYSIOLOGY.
145(4), 1110-1117.
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Qiu, W., & Scholthof, H. B.
(2007).
Using vectors derived from tomato bushy stunt virus (TBSV) and TBSV defective interfering RNAs (DIs). Current protocols in microbiology.
Chapter 16(1), Unit-16I.4.
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Omarov, R. T., Ciomperlik, J. J., & Scholthof, H. B.
(2007).
RNAi-associated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex. Proceedings of the National Academy of Sciences of the United States of America.
104(5), 1714-1719.
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Scholthof, H. B.
(2006).
The Tombusvirus-encoded P19: from irrelevance to elegance. Nature Reviews Microbiology.
4(5), 405-411.
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Omarov, R., Sparks, K., Smith, L., Zindovic, J., & Scholthof, H. B.
(2006).
Biological relevance of a stable biochemical interaction between the tombusvirus-encoded P19 and short interfering RNAs. Journal of Virology.
80(6), 3000-3008.
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Skare, J. M., Wijkamp, I., Denham, I., Rezende, J., Kitajima, E. W., Park, J., ... Scholthof, H. B.
(2006).
A new eriophyid mite-borne membrane-enveloped virus-like complex isolated from plants. Virology.
347(2), 343-353.
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Yamamura, Y., & Scholthof, H. B.
(2005).
Tomato bushy stunt virus: a resilient model system to study virus-plant interactions. Molecular Plant Pathology.
6(5), 491-502.
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Scholthof, H. B.
(2005).
Plant virus transport: motions of functional equivalence. Trends in Plant Science.
10(8), 376-382.
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Park, J., Faure-Rabasse, S., Robinson, M. A., Desvoyes, B., & Scholthof, H. B.
(2004).
The multifunctional plant viral suppressor of gene silencing P19 interacts with itself and an RNA binding host protein. 323(1), 49-58.
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Omarov, R. T., Rezende, J., & Scholthof, H. B.
(2004).
Host-specific generation and maintenance of Tomato bushy stunt virus defective interfering RNAs. Molecular Plant-Microbe Interactions.
17(2), 195-201.
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Skare, J. M., Wijkamp, I., Rezende, J., Michels, G., Rush, C., Scholthof, K., & Scholthof, H. B.
(2003).
Colony establishment and maintenance of the eriophyid wheat curl mite Aceria tosichella for controlled transmission studies on a new virus-like pathogen. Journal of Virological Methods.
108(1), 133-137.
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Turina, M., Omarov, R., Murphy, J. F., Bazaldua-Hernandez, C., Desvoyes, B., & Scholthof, H. B.
(2003).
A newly identified role for Tomato bushy stunt virus P19 in short distance spread. Molecular Plant Pathology.
4(1), 67-72.
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Desvoyes, B., & Scholthof, H. B.
(2002).
Host-dependent recombination of a Tomato bushy stunt virus coat protein mutant yields truncated capsid subunits that form virus-like complexes which benefit systemic spread. 304(2), 434-442.
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Park, J., Desvoyes, B., & Scholthof, H. B.
(2002).
Tomato bushy stunt virus genomic RNA accumulation is regulated by interdependent cis-acting elements within the movement protein open reading frames. Journal of Virology.
76(24), 12747-12757.
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Desvoyes, B., Faure-Rabasse, S., Chen, M., Park, J., & Scholthof, H. B.
(2002).
A novel plant homeodomain protein interacts in a functionally relevant manner with a virus movement protein. PLANT PHYSIOLOGY.
129(4), 1521-1532.
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Qiu, W., Park, J., & Scholthof, H. B.
(2002).
Tombusvirus P19-mediated suppression of virus-induced gene silencing is controlled by genetic and dosage features that influence pathogenicity. Molecular Plant-Microbe Interactions.
15(3), 269-280.
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Scholthof, K., Mirkov, T. E., & Scholthof, H. B.
(2002).
Plant virus gene vectors: biotechnology applications in agriculture and medicine. Genetic engineering.
24, 67-85.
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Qiu, W., & Scholthof, H. B.
(2001).
Effects of inactivation of the coat protein and movement genes of Tomato bushy stunt virus on early accumulation of genomic and subgenomic RNAs. JOURNAL OF GENERAL VIROLOGY.
82(Pt 12), 3107-3114.
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Scholthof, H. B.
(2001).
Molecular Plant-Microbe Interactions That Cut the Mustard. PLANT PHYSIOLOGY.
127(4), 1476-1483.
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Scholthof, H. B.
(2001).
Molecular plant-microbe interactions that cut the mustard. 127(4), 1476-1483.
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Bringmann, A., Schopf, S., Faude, F., & Reichenbach, A.
(2001).
Arachidonic acid-induced inhibition of Ca2+ channel currents in retinal glial (Mller) cells. Graefes Archive for Clinical and Experimental Ophthalmology.
239(11), 859-864.
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Qiu, W., Park, J. W., Jackson, A. O., & Scholthof, H. B.
(2001).
Retention of a small replicase gene segment in tomato bushy stunt virus defective RNAs inhibits their helper-mediated trans-accumulation. 281(1), 51-60.
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Qiu, W., & Scholthof, H. B.
(2001).
Effects of inactivation of the coat protein and movement genes of Tomato bushy stunt virus on early accumulation of genomic and subgenomic RNAs. JOURNAL OF GENERAL VIROLOGY.
82(12), 3107-3114.
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Chu, M., Desvoyes, B., Turina, M., Noad, R., & Scholthof, H. B.
(2000).
Genetic dissection of tomato bushy stunt virus p19-protein-mediated host-dependent symptom induction and systemic invasion. Virology.
266(1), 79-87.
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Scholthof, H. B.
(1999).
Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector. Journal of Virology.
73(9), 7823-7829.
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Scholthof, H. B., Desvoyes, B., Kuecker, J., & Whitehead, E.
(1999).
Biological activity of two tombusvirus proteins translated from nested genes is influenced by dosage control via context-dependent leaky scanning. Molecular Plant-Microbe Interactions.
12(8), 670-679.
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Ranu, R. S., Scholthof, H. B., Gowda, S., & Shepherd, R. J.
(1999).
Synthesis of figwort mosaic virus (FMV) coat protein-reverse transcriptase fusion protein in reticulocyte lysates from a full-length transcript. Journal of Plant Biochemistry and Biotechnology.
8(2), 81-85.
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Rubio, T., Borja, M., Scholthof, H. B., Feldstein, P. A., Morris, T. J., & Jackson, A. O.
(1999).
Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants. Journal of Virology.
73(6), 5070-5078.
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Chu, M., Park, J. W., & Scholthof, H. B.
(1999).
Separate regions on the tomato bushy stunt virus p22 protein mediate cell-to-cell movement versus elicitation of effective resistance responses. Molecular Plant-Microbe Interactions.
12(4), 285-292.
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Rubio, T., Borja, M., Scholthof, H. B., & Jackson, A. O.
(1999).
Recombination with host transgenes and effects on virus evolution: An overview and opinion. Molecular Plant-Microbe Interactions.
12(2), 87-92.
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Borja, M., Rubio, T., Scholthof, H. B., & Jackson, A. O.
(1999).
Restoration of wild-type virus by double recombination of tombusvirus mutants with a host transgene. Molecular Plant-Microbe Interactions.
12(2), 153-162.
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Creager, A. N., Scholthof, K. B., Citovsky, V., & Scholthof, H. B.
(1999).
Tobacco mosaic virus. Pioneering research for a century. The Plant Cell.
11(3), 301-308.
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Scholthof, H. B., & Jackson, A. O.
(1997).
The enigma of pX: A host-dependent cis-acting element with variable effects on tombusvirus RNA accumulation. Virology.
237(1), 56-65.
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Blanc, S., Schmidt, I., Vantard, M., Scholthof, H. B., Kuhl, G., Esperandieu, P., Cerutti, M., & Louis, C.
(1997).
Aphids, viruses and the cytoskeleton. Trends in Plant Science.
2(4), 123.
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Ranu, R. S., Gowda, S., Scholthof, H., Wu, F. C., & Shepherd, R. J.
(1996).
In vitro translation of the full-length RNA transcript of figwort mosaic virus (Caulimovirus). Gene Expression: International Journal of Molecular and Cellular Science.
5(3), 143-153.
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Ranu, R., Scholthof, H., Fiyda, S. S., & Shepherd, R. J.
(1996).
Synthesis of figwort mosaic virus (FMV) coat protein - reverse transcriptase fusion protein in reticulocyte lysate from a full length transcript. The FASEB Journal.
10(6),
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Blanc, S., Schmidt, I., Vantard, M., Scholthof, H. B., Kuhl, G., Esperandieu, P., Cerutti, M., & Louis, C.
(1996).
The aphid transmission factor of cauliflower mosaic virus forms a stable complex with microtubules in both insect and plant cells. Proceedings of the National Academy of Sciences of the United States of America.
93(26), 15158-15163.
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Scholthof, H. B., Scholthof, K. B., & Jackson, A. O.
(1996).
Plant virus gene vectors for transient expression of foreign proteins in plants. Annual Review of Phytopathology.
34(1), 299-323.
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Scholthof, H. B., Scholthof, K. B., Kikkert, M., & Jackson, A. O.
(1995).
Tomato bushy stunt virus spread is regulated by two nested genes that function in cell-to-cell movement and host-dependent systemic invasion. Virology.
213(2), 425-438.
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Scholthof, K. B., Scholthof, H. B., & Jackson, A. O.
(1995).
The effect of defective interfering RNAs on the accumulation of tomato bushy stunt virus proteins and implications for disease attenuation. Virology.
211(1), 324-328.
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Chang, Y. C., Borja, M., Scholthof, H. B., Jackson, A. O., & Morris, T. J.
(1995).
Host effects and sequences essential for accumulation of defective interfering RNAs of cucumber necrosis and tomato bushy stunt tombusviruses. Virology.
210(1), 41-53.
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Scholthof, K. B., Scholthof, H. B., & Jackson, A. O.
(1995).
The tomato bushy stunt virus replicase proteins are coordinately expressed and membrane associated. Virology.
208(1), 365-369.
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Scholthof, H. B., Scholthof, K. B., & Jackson, A. O.
(1995).
Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. The Plant Cell.
7(8), 1157-1172.
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Scholthof, K., Scholthof, H. B., & Jackson, A. O.
(1993).
Control of Plant Virus Diseases by Pathogen-Derived Resistance in Transgenic Plants. PLANT PHYSIOLOGY.
102(1), 7-12.
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Scholthof, H. B., Wu, F. C., Kiernan, J. M., & Shepherd, R. J.
(1993).
The putative zinc finger of a caulimovirus is essential for infectivity but does not influence gene expression. JOURNAL OF GENERAL VIROLOGY.
74 ( Pt 4)(4), 775-780.
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Scholthof, H. B., Wu, F. C., Gowda, S., & Shepherd, R. J.
(1992).
Regulation of caulimovirus gene expression and the involvement of cis-acting elements on both viral transcripts. Virology.
190(1), 403-412.
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Scholthof, H. B., Gowda, S., Wu, F. C., & Shepherd, R. J.
(1992).
The full-length transcript of a caulimovirus is a polycistronic mRNA whose genes are trans activated by the product of gene VI. Journal of Virology.
66(5), 3131-3139.
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Gowda, S., Scholthof, H. B., Wu, F. C., & Shepherd, R. J.
(1991).
Requirement of gene VII in cis for the expression of downstream genes on the major transcript of figwort mosaic virus. Virology.
185(2), 867-871.
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Scholthof, H. B., Wu, F. C., Richins, R. D., & Shepherd, R. J.
(1991).
A naturally occurring deletion mutant of figwort mosaic virus (caulimovirus) is generated by RNA splicing. Virology.
184(1), 290-298.
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Gowda, S., Wu, F. C., Scholthof, H. B., & Shepherd, R. J.
(1989).
Gene VI of figwort mosaic virus (caulimovirus group) functions in posttranscriptional expression of genes on the full-length RNA transcript. Proceedings of the National Academy of Sciences of the United States of America.
86(23), 9203-9207.
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Richins, R. D., Scholthof, H. B., & Shepherd, R. J.
(1987).
Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Research (NAR).
15(20), 8451-8466.
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DeMell, A., Mendoza, M., & Scholthof, H. B.
A Tomato bushy stunt virus-based vector for simultaneous editing and sensing to survey the host antiviral RNA silencing machinery. pgad436.
-
DeMell, A., Alvarado, V., & Scholthof, H. B.
(2023).
Molecular perspectives on age-related resistance of plants to (viral) pathogens. New Phytologist.
-
conference paper
-
Abeuova, L. S., Scholthof, H. B., Ramankulov, E. M., & Manabayeva, S. A.
(2015).
Tomato bushy stunt virus-based vector for transient expression of human G-CSF in Nicotiana benthamiana plants. Journal of Biotechnology.
S29-S29.
-
Makenova, A. T., Scholthof, H. B., Ramankulov, E. M., & Manabayeva, S. A.
(2015).
Transient expression of Acidothermus cellulolyticus endoglucanase E1 by a Tomato bushy stunt virus-based plant expression vector. Journal of Biotechnology.
S29-+.
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Manabayeva, S. A., & Scholthof, H. B.
(2013).
Heterologous expression of a viral coat protein facilitates long-distance movement of a recombinant protein expressing virus vector. Current Opinion in Biotechnology.
S35-S36.
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Abeuova, L. S., Scholthof, H. B., Ramankulov, E. M., & Manabayeva, S. A.
(2015).
Tomato bushy stunt virus-based vector for transient expression of human G-CSF in Nicotiana benthamiana plants. Journal of Biotechnology.
S29-S29.
-
institutional repository document
-
Cody, W. B., & Scholthof, H. B.
(2020).
Native ribonucleases process sgRNA transcripts to create catalytic Cas9/sgRNA complexes in planta
-
Cody, W. B., & Scholthof, H. B.
(2020).
Native ribonucleases process sgRNA transcripts to create catalytic Cas9/sgRNA complexes in planta
Research
principal investigator on
- Developing viral-based gene editing tools to probe antiviral RNA silencing in plants awarded by National Institute of Food and Agriculture - (Washington D.C., District of Columbia, United States) 2018 - 2023
- Plant-Virus interactions that control antiviral RNA silencing awarded by United States Department of Agriculture - (Washington D.C., District of Columbia, United States) 2015 - 2019
- Application Of A Viral Vector For R Gene Interrogation In Nicotiana Tabacum awarded by United States Department of Agriculture - (Washington D.C., District of Columbia, United States) 2017 - 2018
Teaching
teaching activities
- BESC481 Seminar Instructor
- BESC484 Field Experience Instructor
- BESC484 Field Experience Instructor
- PLPA605 Molecular Plant Virology Instructor
- PLPA616 Molecular Bio Pl-microbe Instructor
- PLPA691 Research Instructor
- PLPA691 Research Instructor
Works By Students
chaired theses and dissertations
-
Ciomperlik, Jessica J. (2010-01). Anti-viral RNAi and its suppression in plants..
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Herron, Caroline Mary (2004-11). Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies.
-
Hsieh, Yi-Cheng (2010-01). PROPERTIES OF THE TOMBUSVIRUS MOVEMENT PROTEIN AND RNAi SUPPRESSOR THAT INFLUENCE PATHOGENESIS.
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McLachlan, Juanita (2013-04). A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus.
-
Mendoza Hernandez, Maria Regina (2016-04). Argonaute 2 and Antiviral Silencing in Plants.
-
Odokonyero, Denis 1984- (2012-12). Identification of ARGONAUTES Involved in Antiviral RNA Silencing in Nicotiana benthamiana.
-
Seaberg, Bonnie Lee (2009-05). Host factors involved in viral movement through plants.
Background
education and training
- Ph.D. in Plant Pathology, University of Kentucky - (Lexington, Kentucky, United States) 1990
awards and honors
- Fellow, conferred by American Phytopathological Society - (Saint Paul, Minnesota, United States), 2009
- Ruth Allen Award, conferred by American Phytopathological Society - (Saint Paul, Minnesota, United States), 2007
Contact
first name
- Herman
has last name
- Scholthof
mailing address
-
Texas A&M University
Plant Pathology & Microbiology
2132 TAMU
College Station, TX 77843-2132
USA
Other
- herscho@tamu.edu