Satellite panicum mosaic virus coat protein enhances the performance of plant virus gene vectors. Academic Article

abstract

• The coat protein of satellite panicum mosaic virus (SPCP) is known to effectively protect its cognate RNA from deleterious events, and here, we tested its stabilizing potential for heterologous virus-based gene vectors in planta. In support of this, a Potato virus X (PVX) vector carrying the SPMV capsid protein (PVX-SPCP) gene was stable for at least three serial systemic passages through Nicotiana benthamiana. To test the effect of SPCP in trans, PVX-SPCP was co-inoculated onto N. benthamiana together with a Tomato bushy stunt virus (TBSV) vector carrying a green fluorescent protein (GFP) gene that normally does not support systemic GFP expression. In contrast, co-inoculation of TBSV-GFP plus PVX-SPCP resulted in GFP accumulation and concomitant green fluorescent spots in upper, non-inoculated leaves in a temperature-responsive manner. These results suggest that the multifaceted SPMV CP has intriguing effects on virus-host interactions that surface in heterologous systems.

authors

• Scholthof, Herman
• Scholthof, Karen-Beth

published proceedings

• Virology

author list (cited authors)

• Everett, A. L., Scholthof, H. B., & Scholthof, K.

citation count

• 17

complete list of authors

• Everett, Anthany L||Scholthof, Herman B||Scholthof, Karen-Beth G

publication date

• January 2010

publisher

• Elsevier  Publisher

published in

• Virology  Journal

keywords

• Capsid Proteins
• Genetic Vectors
• Green Fluorescent Proteins
• Mosaic Viruses
• Panicum
• Plant Viruses
• Potexvirus
• Reverse Transcriptase Polymerase Chain Reaction
• Satellite Viruses
• Temperature
• Tombusvirus

Digital Object Identifier (DOI)

• 10.1016/j.virol.2009.10.006

start page

• 37

end page

• 46

volume

• 396

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.virol.2009.10.006