Neural Stem Cell or Human Induced Pluripotent Stem Cell-Derived GABA-ergic Progenitor Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy.
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Grafting of neural stem cells (NSCs) or GABA-ergic progenitor cells (GPCs) into the hippocampus could offer an alternative therapy to hippocampal resection in patients with drug-resistant chronic epilepsy, which afflicts >30% of temporal lobe epilepsy (TLE) cases. Multipotent, self-renewing NSCs could be expanded from multiple regions of the developing and adult brain, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiPSCs). On the other hand, GPCs could be generated from the medial and lateral ganglionic eminences of the embryonic brain and from hESCs and hiPSCs. To provide comprehensive methodologies involved in testing the efficacy of transplantation of NSCs and GPCs in a rat model of chronic TLE, NSCs derived from the rat medial ganglionic eminence (MGE) and MGE-like GPCs derived from hiPSCs are taken as examples in this unit. The topics comprise description of the required materials, reagents and equipment, methods for obtaining rat MGE-NSCs and hiPSC-derived MGE-like GPCs in culture, generation of chronically epileptic rats, intrahippocampal grafting procedure, post-grafting evaluation of the effects of grafts on spontaneous recurrent seizures and cognitive and mood impairments, analyses of the yield and the fate of graft-derived cells, and the effects of grafts on the host hippocampus. © 2016 by John Wiley & Sons, Inc.
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Curr Protoc Stem Cell Biol
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Upadhya, D., Hattiangady, B., Shetty, G. A., Zanirati, G., Kodali, M., & Shetty, A. K.
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Upadhya, Dinesh||Hattiangady, Bharathi||Shetty, Geetha A||Zanirati, Gabriele||Kodali, Maheedhar||Shetty, Ashok K
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keywords
Affect
Animals
Cell Differentiation
Cell Transplantation
Chronic Disease
Chronic Temporal Lobe Epilepsy
Cognition
Cognitive And Mood Impairments
Disease Models, Animal
Epilepsy, Temporal Lobe
Female
GABAergic Neurons
Gaba-ergic Progenitors
Glial-cell Line Derived Neurotrophic Factor
Hippocampal Neurogenesis
Hippocampus
Human Induced Pluripotent Stem Cells
Humans
Induced Pluripotent Stem Cells
Male
Medial Ganglionic Eminence
Median Eminence
Neural Stem Cells
Phenotypic Differentiation Of Graft-derived Cells
Rats, Inbred F344
Spontaneous Recurrent Seizures
Stem Cell Transplantation
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