HR job title
- Professor
- Contact Info
- 1 979 845 6312
- Websites
Igumenova, Tatyana Faculty Member
Positions
- Associate Professor, Biochemistry and Biophysics, College of Agriculture and Life Sciences
My laboratory is broadly interested in understanding the structural basis of signal transduction events that occur at the membrane surface. These events are mediated by signaling proteins that reversibly associate with membranes in response to binding second messengers, such as Ca2+ ions, diacylglycerol, and phosphoinositides. One of the key kinases regulating these signal transduction pathways is the Protein Kinase C (PKC) family. Aberrant levels of PKC expression or activity have been implicated in a large number of human diseases, such as cancer, cardiac failure, Alzheimer's disease, and diabetes. Despite the significance of PKC in signal transduction and human health, the structural and dynamical basis of its activation upon binding to lipid membranes remains elusive.
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Overview
Publications
selected publications
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academic article
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Chen, X., Dixit, K., Yang, Y., McDermott, M. I., Imam, H. T., Bankaitis, V. A., & Igumenova, T. I.
(2024).
A novel bivalent interaction mode underlies a non-catalytic mechanism for Pin1-mediated protein kinase C regulation. eLife.
13, e92884.
-
Chen, X., & Igumenova, T. I.
(2023).
Regulation of eukaryotic protein kinases by Pin1, a peptidyl-prolyl isomerase. 87, 100938-100938.
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Chen, X., & Igumenova, T. I.
(2022).
Detection of the Pin1-ligand interactions using 19F NMR spectroscopy. The FASEB Journal.
36(S1),
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Katti, S. S., Krieger, l. V., Sacchettini, J. C., & Igumenova, T. I.
(2022).
Structural Basis of Agonist Capture by Regulatory Cl Domain of PKC. 36(S1),
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Katti, S. S., Krieger, I. V., Poudel, L., Sacchettini, J. S., & Igumenova, T. I.
(2022).
Mechanism of diacylglycerol sensing revealed by integrative structural biology approach. Biophysical Journal.
121(3), 224A-224A.
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Bankaitis, V. A., Tripathi, A., Chen, X., & Igumenova, T. I.
(2022).
New strategies for combating fungal infections: Inhibiting inositol lipid signaling by targeting Sec14 phosphatidylinositol transfer proteins. Advances in Biological Regulation.
84, 100891-100891.
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Katti, S. S., Krieger, I. V., Ann, J., Lee, J., Sacchettini, J. C., & Igumenova, T. I.
(2022).
Structural anatomy of Protein Kinase C C1 domain interactions with diacylglycerol and other agonists. Nature Communications.
13(1), 2695.
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Katti, S. S., Krieger, I. V., Sacchettini, J. C., & Igumenova, T. I.
(2022).
Structural basis of agonist capture by PKC-regulatory C1 domain. Biophysical Journal.
121(3), 224A-224A.
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You, Y., Katti, S., Yu, B., Igumenova, T. I., & Das, J.
(2021).
Probing the Diacylglycerol Binding Site of Presynaptic Munc13-1. Biochemistry.
60(16), 1286-1298.
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Cole, T. R., & Igumenova, T. I.
(2021).
Reactivity of Thiol-Rich Zn Sites in Diacylglycerol-Sensing PKC C1 Domain Probed by NMR Spectroscopy. Frontiers in Molecular Biosciences.
8, 728711.
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Katti, S., & Igumenova, T. I.
(2021).
Structural insights into C1-ligand interactions: Filling the gaps by in silico methods. Advances in Biological Regulation.
79, 100784-100784.
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Katti, S., Nyenhuis, S. B., Her, B., Cafiso, D. S., & Igumenova, T. I.
(2020).
Partial Metal Ion Saturation of C2 Domains Primes Synaptotagmin 1-Membrane Interactions. Biophysical Journal.
118(6), 1409-1423.
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Katti, S., & Igumenova, T. I.
(2020).
Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity?. Metallomics.
12(2), 164-172.
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Taylor, J. T., Mukherjee, P. K., Puckhaber, L. S., Dixit, K., Igumenova, T. I., Suh, C., Horwitz, B. A., & Kenerley, C. M.
(2020).
Deletion of the Trichoderma virens NRPS, Tex7, induces accumulation of the anti-cancer compound heptelidic acid. Biochemical and Biophysical Research Communications.
529(3), 672-677.
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Cole, T. R., Erickson, S. G., Morales, K. A., Sung, M., Holzenburg, A., & Igumenova, T. I.
(2019).
Cd(II)- and Pb(II)-Induced Self-Assembly of Peripheral Membrane Domains from Protein Kinase C. Biochemistry.
58(6), 509-513.
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You, Y., Yu, B., Igumenova, T., & Das, J.
(2019).
Lipid Membrane Influences Interaction between the C1 Domain of Munc13-1 and the Activator. Biophysical Journal.
116(3), 517a-517a.
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Katti, S., Her, B., Srivastava, A. K., Taylor, A. B., Lockless, S. W., & Igumenova, T. I.
(2018).
High affinity interactions of Pb2+ with synaptotagmin I. Metallomics.
10(9), 1211-1222.
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Yang, Y., Shu, C., Li, P., & Igumenova, T. I.
(2018).
Structural Basis of Protein Kinase C Regulation by the C-Terminal Tail. Biophysical Journal.
114(7), 1590-1603.
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Katti, S., Nyenhuis, S. B., Her, B., Srivastava, A. K., Taylor, A. B., Hart, P. J., Cafiso, D. S., & Igumenova, T. I.
(2017).
Non-Native Metal Ion Reveals the Role of Electrostatics in Synaptotagmin 1-Membrane Interactions. Biochemistry.
56(25), 3283-3295.
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Stewart, M. D., & Igumenova, T. I.
(2017).
Toggling of Diacylglycerol Affinity Correlates with Conformational Plasticity in C1 Domains. Biochemistry.
56(21), 2637-2640.
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Morales, K. A., Yang, Y., Cole, T. R., & Igumenova, T. I.
(2016).
Dynamic Response of the C2 Domain of Protein Kinase C to Ca2+ Binding. Biophysical Journal.
111(8), 1655-1667.
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Igumenova, T. I.
(2015).
Dynamics and Membrane Interactions of Protein Kinase C. Biochemistry.
54(32), 4953-4968.
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Cole, T. R., & Igumenova, T. I.
(2015).
Expression and purification of the N-terminal regulatory domain of Protein Kinase C for biophysical studies. Protein Expression and Purification.
110, 14-21.
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Ghosh, R., de Campos, M., Huang, J., Huh, S. K., Orlowski, A., Yang, Y., ... Bankaitis, V. A.
(2015).
Sec14-nodulin proteins and the patterning of phosphoinositide landmarks for developmental control of membrane morphogenesis. 26(9), 1764-1781.
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Yang, Y., Morales, K. A., Stewart, M. D., & Igumenova, T. I.
(2015).
Conditional Membrane Proteins: Solution NMR Studies of Structure, Dynamics, and Function. eMagRes.
4(4), 767-777.
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Stewart, M. D., Cole, T. R., & Igumenova, T. I.
(2014).
Interfacial partitioning of a loop hinge residue contributes to diacylglycerol affinity of conserved region 1 domains. 289(40), 27653-27664.
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Morales, K. A., Yang, Y., Long, Z., Li, P., Taylor, A. B., Hart, P. J., & Igumenova, T. I.
(2013).
Cd2+ as a Ca2+ surrogate in protein-membrane interactions: isostructural but not isofunctional. Journal of the American Chemical Society.
135(35), 12980-12983.
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Yang, Y., & Igumenova, T. I.
(2013).
The C-terminal V5 domain of Protein Kinase C is intrinsically disordered, with propensity to associate with a membrane mimetic. PLoS ONE.
8(6), e65699-e65699.
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Stewart, M. D., & Igumenova, T. I.
(2012).
Reactive cysteine in the structural Zn(2+) site of the C1B domain from PKC. Biochemistry.
51(37), 7263-7277.
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Morales, K. A., & Igumenova, T. I.
(2012).
Synergistic effect of Pb(2+) and phosphatidylinositol 4,5-bisphosphate on C2 domain-membrane interactions. Biochemistry.
51(16), 3349-3360.
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Shu, C., Sung, M. W., Stewart, M. D., Igumenova, T. I., Tan, X., & Li, P.
(2012).
The structural basis of iron sensing by the human F-box protein FBXL5. ChemBioChem.
13(6), 788-791.
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Morales, K. A., Lasagna, M., Gribenko, A. V., Yoon, Y., Reinhart, G. D., Lee, J. C., ... Igumenova, T. I.
(2011).
Pb2+ as modulator of protein-membrane interactions. Journal of the American Chemical Society.
133(27), 10599-10611.
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Stewart, M. D., Morgan, B., Massi, F., & Igumenova, T. I.
(2011).
Probing the determinants of diacylglycerol binding affinity in the C1B domain of protein kinase C. Journal of Molecular Biology.
408(5), 949-970.
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Li, X., Lu, C., Stewart, M., Xu, H., Strong, R. K., Igumenova, T., & Li, P.
(2009).
Structural basis of double-stranded RNA recognition by the RIG-I like receptor MDA5. Archives of Biochemistry and Biophysics.
488(1), 23-33.
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Igumenova, T. I., Brath, U., Akke, M., & Palmer, A. G.
(2007).
Characterization of chemical exchange using residual dipolar coupling. Journal of the American Chemical Society.
129(44), 13396-13397.
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Berlow, R. B., Igumenova, T. I., & Loria, J. P.
(2007).
Value of a hydrogen bond in triosephosphate isomerase loop motion. Biochemistry.
46(20), 6001-6010.
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Igumenova, T. I., & Palmer, A. G.
(2006).
Off-resonance TROSY-selected R 1rho experiment with improved sensitivity for medium- and high-molecular-weight proteins. Journal of the American Chemical Society.
128(25), 8110-8111.
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Igumenova, T. I., Frederick, K. K., & Wand, A. J.
(2006).
Characterization of the fast dynamics of protein amino acid side chains using NMR relaxation in solution. CHEMICAL REVIEWS.
106(5), 1672-1699.
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Igumenova, T. I., Lee, A. L., & Wand, A. J.
(2005).
Backbone and side chain dynamics of mutant calmodulin-peptide complexes. Biochemistry.
44(38), 12627-12639.
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Igumenova, T. I., & McDermott, A. E.
(2005).
Homo-nuclear 13C J-decoupling in uniformly 13C-enriched solid proteins. Journal of Magnetic Resonance.
175(1), 11-20.
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Wang, T., Frederick, K. K., Igumenova, T. I., Wand, A. J., & Zuiderweg, E.
(2005).
Changes in calmodulin main-chain dynamics upon ligand binding revealed by cross-correlated NMR relaxation measurements. Journal of the American Chemical Society.
127(3), 828-829.
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Igumenova, T. I., McDermott, A. E., Zilm, K. W., Martin, R. W., Paulson, E. K., & Wand, A. J.
(2004).
Assignments of carbon NMR resonances for microcrystalline ubiquitin. Journal of the American Chemical Society.
126(21), 6720-6727.
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Igumenova, T. I., Wand, A. J., & McDermott, A. E.
(2004).
Assignment of the backbone resonances for microcrystalline ubiquitin. Journal of the American Chemical Society.
126(16), 5323-5331.
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Igumenova, T. I., & McDermott, A. E.
(2003).
Improvement of resolution in solid state NMR spectra with J-decoupling: an analysis of lineshape contributions in uniformly 13C-enriched amino acids and proteins. Journal of Magnetic Resonance.
164(2), 270-285.
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Igumenova, T. I., Mitchell, D. J., & Evans, J. N.
(1997).
Comparative studies of phase-cycling schemes for multiple pi-pulse sequences. Journal of Magnetic Resonance.
127(2), 144-146.
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Mitchell, D. J., Jakeman, D. L., Igumenova, T. I., Shuttleworth, W. A., Miller, K. D., & Evans, J.
(1997).
Time-resolved solid-state REDOR-edited NMR detection of a transient enzyme-intermediate. Chemical Communications.
0(11), 1019-1020.
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Igumenova, T. I., Vasiltsova, O. V., & Parmon, V. N.
(1996).
Formation and photocatalytic properties of Q-sized nanoparticles of various transition metal sulphides in the inner cavities of lecithin vesicles modified with sodium dodecylsulphate. Journal of Photochemistry and Photobiology A: Chemistry.
94(2-3), 205-213.
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IGUMENOVA, T. I., & PARMON, V. N.
(1995).
PHOTOSTIMULATED VECTORIAL ELECTRON-TRANSFER ACROSS THE BILAYER-MEMBRANE OF LIPID VESICLES IN A SYSTEM WITH CDS NANOPARTICLES AS PHOTOSENSITIZER AND 1,4-BIS(1,2,6-TRIPHENYL-4-PYRIDYL) BENZENE AS A REVERSIBLE 2-ELECTRON CARRIER. 90(2-3), 159-166.
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Chen, X., Dixit, K., Yang, Y., McDermott, M. I., Imam, H. T., Bankaitis, V. A., & Igumenova, T. I.
(2024).
A novel bivalent interaction mode underlies a non-catalytic mechanism for Pin1-mediated protein kinase C regulation. eLife.
13, e92884.
-
conference paper
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Igumenova, T. I., Cole, T. R., Katti, S., Morales, K. A., Erickson, S. G., Sung, M. W., ... Cafiso, D. S.
(2018).
Not Just Ionic Mimicry: Biophysics of Toxic Metal Ion Interactions with Peripheral Membrane Targets. Biophysical Journal.
33A-33A.
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Katti, S., Her, B., Srivastava, A., Taylor, A. B., Hart, P. J., Lockless, S., & Igumenova, T.
(2018).
Structural and Kinetic Characterization of High-Affinity Lead(II)-Synaptotagmin I Interactions. Biophysical Journal.
572A-572A.
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Cole, T. R., Erickson, S. G., Sung, M. W., Holzenburg, A., & Igumenova, T. I.
(2017).
Toxic Metal Ions Promote Self-Association and Replace Structural Zinc Ions in the Regulatory Region of Protein Kinase C. Biophysical Journal.
489A-490A.
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Igumenova, T. I., Cole, T. R., Katti, S., Morales, K. A., Erickson, S. G., Sung, M. W., ... Cafiso, D. S.
(2018).
Not Just Ionic Mimicry: Biophysics of Toxic Metal Ion Interactions with Peripheral Membrane Targets. Biophysical Journal.
33A-33A.
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institutional repository document
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Katti, S., & Igumenova, T. I.
(2020).
Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity?
-
Katti, S., Nyenhuis, S. B., Her, B., Cafiso, D. S., & Igumenova, T. I.
(2019).
Partial metal ion saturation of C2 domains primes Syt1-membrane interactions
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Katti, S., Her, B., Srivastava, A. K., Taylor, A. B., Lockless, S. W., & Igumenova, T. I.
(2018).
High affinity interactions of Pb2+ with Synaptotagmin I
-
Katti, S., & Igumenova, T. I.
(2020).
Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity?
Research
principal investigator on
- Mechanisms of signal transduction revealed through unique chemistry of xenobiotic metal ions awarded by National Science Foundation - (Arlington, Virginia, United States) 2019 - 2022
- Structural and functional studies of protein kinase C regulation awarded by National Institutes of Health - (Bethesda, Maryland, United States) 2014 - 2020
- CAREER: Biological Chemistry of Ph2+ Revealed Through Ph2+ Mediated Protein-Membrane Interactions awarded by National Science Foundation - (Arlington, Virginia, United States) 2012 - 2018
Teaching
teaching activities
- BICH101 Perspectives In Bich And Gene Instructor
- BICH404 Biochemical Calculations Instructor
- BICH491 Hnr-research Instructor
- BICH685 Directed Studies Instructor
- BICH689 Sptp:biological Nmr Spectrosc Instructor
- BICH691 Research Instructor
- GENE101 Perspectives In Bich And Gene Instructor
Works By Students
chaired theses and dissertations
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Morales-Rivera, Krystal A. (2013-11). The Metal-dependent Function of C2?: A Conditional Membrane Domain from Protein Kinase C?.
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Stewart, Mikaela D. (2013-06). Determining the Intrinsic Properties of the C1B Domain that Influence PKC Ligand Specificity and Sensitivity to Reactive Oxygen Species.
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Yang, Yuan (2016-07). Investigating the Roles of the C-terminal Tail and the Metal-dependent C2 Domain in PKCa Regulation.
Background
education and training
awards and honors
Contact
first name
- Tatyana
has last name
- Igumenova
mailing address
-
Texas A&M University
Biochemistry & Biophysics
2128 TAMU
College Station, TX 77843-2128
USA
Other
- tigumenova@tamu.edu