Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity? Academic Article

abstract

• Pb2+ is a xenobiotic metal ion that competes for Ca2+-binding sites in proteins. Using the peripheral Ca2+-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb2+ sites that are likely to be targeted by bioavailable Pb2+.

authors

• Igumenova, Tatyana

published proceedings

• Metallomics

altmetric score

• 0.25

author list (cited authors)

• Katti, S., & Igumenova, T. I.

citation count

• 1

complete list of authors

• Katti, Sachin||Igumenova, Tatyana I

publication date

• February 2020

publisher

• Oxford University Press (OUP)  Publisher

published in

• Metallomics  Journal

keywords

• Binding Sites
• Binding, Competitive
• Buffers
• Calcium
• Cell Membrane
• Hydrogen-Ion Concentration
• Lead
• Protein Binding
• Protein Domains
• Synaptotagmin I
• Xenobiotics

PubMed Central ID

• 32051983

Digital Object Identifier (DOI)

• 10.1039/d0mt00002g

start page

• 164

end page

• 172

volume

• 12

issue

• 2

URL

• http://dx.doi.org/10.1039/d0mt00002g