Interference of pH buffer with Pb 2+ -peripheral domain interactions: obstacle or opportunity? Academic Article uri icon

abstract

  • Pb2+ is a xenobiotic metal ion that competes for Ca2+-binding sites in proteins. Using the peripheral Ca2+-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb2+ sites that are likely to be targeted by bioavailable Pb2+.

altmetric score

  • 0.25

author list (cited authors)

  • Katti, S., & Igumenova, T. I.

citation count

  • 1

publication date

  • January 2020