Interference of pH buffer with Pb 2+ -peripheral domain interactions: obstacle or opportunity?
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Pb2+ is a xenobiotic metal ion that competes for Ca2+-binding sites in proteins. Using the peripheral Ca2+-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb2+ sites that are likely to be targeted by bioavailable Pb2+.
author list (cited authors)
Katti, S., & Igumenova, T. I.