Microchannel Electrophoresis Device for Separation and In Situ Detection of Proteins Academic Article uri icon

abstract

  • A new microchannel electrophoresis device, which can effectively separate and identify proteins from a mixture in a short period of time, has been disclosed and discussed. The device structure and fabrication procedure are presented. The detection of the separated protein is based on the principle that electric resistance of the device increases drastically due to the adsorption of the protein on the detection electrode surface. Each protein corresponds to a unique peak time in the current change rate vs. time curve. Examples of separation and detection of proteins using a low electric field are demonstrated. Separated proteins were confirmed by methods of dye staining and electron spectroscopy for chemical analysis. This new device has a high protein separation speed, and is easy to fabricate and operate. It is potentially applicable to the separation of a wide range of biomolecules or similar types of charged macromolecules.

author list (cited authors)

  • Kuo, Y., & Lee, H. H.

citation count

  • 6

publication date

  • January 2001