Patterning a nanowell sensor biochip for specific and rapid detection of bacteria Academic Article

abstract

• This paper presents the fabrication process of a SEPTIC (SEnsing of Phage-Triggered Ion Cascades) chip, consisting of two Ti contact pads and a 150 nm wide Ti nanowell device on LiNbO 3 substrate. The patterning was fulfilled by combining electron beam lithography, contact photolithography and reactive ion etching. When connected to an external preamplifier and spectrum analyzer, the nanowell can probe nano-scale electric field fluctuations. The use of this chip as nano-scale electric field probe was demonstrated by detecting the transitory ion efflux from bacteria being infected by phage. Our experiment showed that the electric field noise follows 1/f 2 power spectrum when the bacteria are sensitive to the phage, whereas 1/f noise corresponds to bacteria resistant to the phage. The use of fluctuation-enhanced sensing can provide sensitivity orders of magnitude higher than conventional sensing that detects changes only in the mean value of electrical signals. 2008.

authors

• King, Maria
• Kish, Laszlo
• Young, Ryland

published proceedings

• MICROELECTRONIC ENGINEERING

altmetric score

• 3

author list (cited authors)

• Seo, S., Dobozi-King, M., Young, R. F., Kish, L. B., & Cheng, M.

citation count

• 25

complete list of authors

• Seo, Sungkyu||Dobozi-King, Maria||Young, Ryland F||Kish, Laszlo B||Cheng, Mosong

publication date

• January 2008

publisher

• Elsevier  Publisher

published in

• Microelectronic Engineering  Journal

keywords

• Bacteria
• Bacteriophage
• Biochip
• Electric Field Fluctuation
• Ion Efflux
• Lithography
• Nanofabrication
• Nanowell
• Power Spectrum
• Reactive Ion Etching

Digital Object Identifier (DOI)

• 10.1016/j.mee.2007.12.046

start page

• 1484

end page

• 1489

volume

• 85

issue

• 7

URL

• http://dx.doi.org/10.1016/j.mee.2007.12.046