Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense. Academic Article uri icon


  • The phagocytosis and destruction of pathogens in lysosomes constitute central elements of innate immune defense. Here, we show that Brucella, the causative agent of brucellosis, the most prevalent bacterial zoonosis globally, subverts this immune defense pathway by activating regulated IRE1-dependent decay (RIDD) of Bloc1s1 mRNA encoding BLOS1, a protein that promotes endosome-lysosome fusion. RIDD-deficient cells and mice harboring a RIDD-incompetent variant of IRE1 were resistant to infection. Inactivation of the Bloc1s1 gene impaired the ability to assemble BLOC-1-related complex (BORC), resulting in differential recruitment of BORC-related lysosome trafficking components, perinuclear trafficking of Brucella-containing vacuoles (BCVs), and enhanced susceptibility to infection. The RIDD-resistant Bloc1s1 variant maintains the integrity of BORC and a higher-level association of BORC-related components that promote centrifugal lysosome trafficking, resulting in enhanced BCV peripheral trafficking and lysosomal destruction, and resistance to infection. These findings demonstrate that host RIDD activity on BLOS1 regulates Brucella intracellular parasitism by disrupting BORC-directed lysosomal trafficking. Notably, coronavirus murine hepatitis virus also subverted the RIDD-BLOS1 axis to promote intracellular replication. Our work establishes BLOS1 as a novel immune defense factor whose activity is hijacked by diverse pathogens.

published proceedings

  • Elife

altmetric score

  • 1.75

author list (cited authors)

  • Wells, K. M., He, K., Pandey, A., Cabello, A., Zhang, D., Yang, J., ... de Figueiredo, P.

citation count

  • 0

complete list of authors

  • Wells, Kelsey Michelle||He, Kai||Pandey, Aseem||Cabello, Ana||Zhang, Dongmei||Yang, Jing||Gomez, Gabriel||Liu, Yue||Chang, Haowu||Li, Xueqiang||Zhang, Hao||Feng, Xuehuang||da Costa, Luciana Fachini||Metz, Richard||Johnson, Charles D||Martin, Cameron Lee||Skrobarczyk, Jill||Berghman, Luc R||Patrick, Kristin L||Leibowitz, Julian||Ficht, Allison||Sze, Sing-Hoi||Song, Jianxun||Qian, Xiaoning||Qin, Qing-Ming||Ficht, Thomas A||de Figueiredo, Paul

publication date

  • May 2022


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