Disulfide bonds are required for Serratia marcescens nuclease activity. Academic Article

abstract

• The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

authors

• Benedik, Michael

published proceedings

• Nucleic Acids Res

author list (cited authors)

• Ball, T. K., Suh, Y., & Benedik, M. J.

citation count

• 33

complete list of authors

• Ball, TK||Suh, Y||Benedik, MJ

publication date

• October 1992

publisher

• Oxford University Press (OUP)  Publisher

published in

• Nucleic Acids Research (NAR)  Journal

keywords

• Alleles
• Blotting, Western
• Cloning, Molecular
• Cysteine
• Disulfides
• Endodeoxyribonucleases
• Endonucleases
• Endoribonucleases
• Genes, Bacterial
• Kinetics
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Plasmids
• Recombinant Proteins
• Serratia Marcescens

PubMed Central ID

• 1329033

Digital Object Identifier (DOI)

• 10.1093/nar/20.19.4971

start page

• 4971

end page

• 4974

volume

• 20

issue

• 19

URL

• http://dx.doi.org/10.1093/nar/20.19.4971