Turmeric curcuminoids (Curcuma longa L.) interacts with microRNAs in the regulation of inflammation on Human Umbilical Vein Endothelial Cells (HUVEC)
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abstract
The antiinflammatory properties of turmeric have been wellinvestigated; however, the involvement of miRNAs in antiinflammatory properties of natural compounds has not been much investigated yet. We studied the potential protective effects of standardized curcuminoid extract (SCE) against vascular inflammation induced by lipopolysacharide (LPS) in HUVEC and investigated underlying miRNA based mechanisms. We screened miRNAs widely express on endothelial cells (miRNA 146a, miRNA 130, miRNA 126a and miRNA 155). However, only miRNA 146 clearly responded to LPS and reversal of this effect by SCE (2.5 20 mg/L) down to 0.4 fold. miRNA 146 might be in control of Tolllike receptor through a negative loop feedback regulation of NFKB activation. We analyzed the effect of SCE on NFKB expression and depended genes within the TLR4pathway which are target of miRNA146. SCE decreased the expression of NFKB, TRAF, IRAK1 and TLR4 by 0.6, 3.5, 1.6, and 0.84fold respectively. When cells were transfected with the mimic of miRNA146, SCE reversed the mimicinduced expression of miRNA146 by 3fold. This indicates that the antiinflammatory effects of SCE are at least in part be exerted through the decrease in miRNA 146. Overall, curcuminoids showed antiinflammatory properties in HUVEC where a decreased of miR146a expression was involved in the underlying antiinflammatory mechanism.Grant Funding Source: ASN
