Standardized Curcuminoids Extract (Curcuma longa L.) Protects against inflammation in Human Umbilical Vein Endothelial Cells (HUVEC) Academic Article uri icon


  • Vascular inflammation is suggested to play a keyrole in the initiation and progression of atherosclerosis. Turmeric, used as medicinal agent in India, contains polyphenolics known as curcuminoids, including curcumin, demethoxycurcumin, and bisdemethoxycurcumin. This study investigated the potential protective effects of standardized curcuminoid extract (SCE) against vascular inflammation induced by high glucose in human umbilical vein endothelial cells (HUVEC). SCE was characterized by HPLCPDA. In this study, high glucose (Glc) (25 mM) increased vascular cell adhesion1 (VCAM) interleukin (IL8), tumor necrosis factor (TNF) and nuclear factor kappa B (NFkB) expression up to 2.1, 1.9, 1.7 and 3.0 fold, respectively, compared to the control and treatment with SCE for 24 h inhibited their mRNA expression by up to 50, 70, 40 and 60%, respectively in a dose dependent manner (025 mg/L), these effects were not due to decreased cell viability as assessed by the MTT test. SCE reversed Glcand lypopolysacharide (LPS) induced increase of microRNA 146a, the NFkBdependent gene up to levels found in control cells (6.2525 mg/L).In addition, treatment with SCE for 1h incubation at 25 and 50 mg/L blocked formation of Glcinduced oxygen species (ROS) by 50 and 70% respectively. The present data suggest that SCE could suppress high glucose induced vascular inflammation generation of ROS in HUVEC.Grant Funding SourceTexas A&M University

published proceedings


author list (cited authors)

  • Angel-Morales, G., Noratto, G., & Mertens-Talcott, S.

citation count

  • 0

complete list of authors

  • Angel-Morales, Gabriela||Noratto, Giuliana||Mertens-Talcott, Susanne

publication date

  • January 2009