Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Academic Article

abstract

• Transfer of the broad-host-range resistance plasmid pIP501 from Streptococcus faecalis to Pediococcus pentosaceus and Pediococcus acidilactici occurred between cells immobilized on nitrocellulose filters in the presence of DNase. Expression of the pIP501-linked erythromycin and chloramphenicol resistance determinants was observed in transconjugants. Intrageneric transfer of pIP501 from a P. pentosaceus donor to various pediococcal recipients occurred at frequencies of 10(-4) to 10(-7) transconjugants per input donor cell. Intergeneric transfer of plasmid pIP501 from P. pentosaceus to S. faecalis, Streptococcus sanguis (Challis), and Streptococcus lactis was observed. Similar mating experiments showed no evidence for the transfer of the broad-host-range R-plasmid pAM beta 1 to Pediococcus spp. recipients.

authors

• Gonzalez, Carlos

published proceedings

• Appl Environ Microbiol

altmetric score

• 3

author list (cited authors)

• Gonzalez, C. F., & Kunka, B. S.

citation count

• 66

complete list of authors

• Gonzalez, CF||Kunka, BS

publication date

• July 1983

publisher

• American Society for Microbiology  Publisher

published in

• Applied and Environmental Microbiology  Journal

keywords

• Anti-Bacterial Agents
• Conjugation, Genetic
• DNA Restriction Enzymes
• DNA, Bacterial
• DNA, Circular
• Pediococcus
• R Factors
• Streptococcus

PubMed Central ID

• 6311098

Digital Object Identifier (DOI)

• 10.1128/aem.46.1.81-89.1983

start page

• 81

end page

• 89

volume

• 46

issue

• 1

URL

• http://dx.doi.org/10.1128/aem.46.1.81-89.1983