Suppression of NLRX1 in chronic obstructive pulmonary disease.
Additional Document Info
Cigarette smoke (CS) and viruses promote the inflammation and remodeling associated with chronic obstructive pulmonary disease (COPD). The MAVS/RIG-I-like helicase (MAVS/RLH) pathway and inflammasome-dependent innate immune pathways are important mediators of these responses. At baseline, the MAVS/RLH pathway is suppressed, and this inhibition must be reversed to engender tissue effects; however, the mechanisms that mediate activation and repression of the pathway have not been defined. In addition, the regulation and contribution of MAVS/RLH signaling in CS-induced inflammation and remodeling responses and in the development of human COPD remain unaddressed. Here, we demonstrate that expression of NLRX1, which inhibits the MAVS/RLH pathway and regulates other innate immune responses, was markedly decreased in 3 independent cohorts of COPD patients. NLRX1 suppression correlated directly with disease severity and inversely with pulmonary function, quality of life, and prognosis. In murine models, CS inhibited NLRX1, and CS-induced inflammation, alveolar destruction, protease induction, structural cell apoptosis, and inflammasome activation were augmented in NLRX1-deficient animals. Conversely, MAVS deficiency abrogated this CS-induced inflammation and remodeling. Restoration of NLRX1 in CS-exposed animals ameliorated alveolar destruction. These data support a model in which CS-dependent NLRX1 inhibition facilitates MAVS/RHL activation and subsequent inflammation, remodeling, protease, cell death, and inflammasome responses.
Kang, M., Yoon, C. M., Kim, B. H., Lee, C., Zhou, Y., Sauler, M., ... Elias, J. A.
complete list of authors
Kang, Min-Jong||Yoon, Chang Min||Kim, Bo Hye||Lee, Chang-Min||Zhou, Yang||Sauler, Maor||Homer, Rober||Dhamija, Anish||Boffa, Daniel||West, Andrew Phillip||Shadel, Gerald S||Ting, Jenny P||Tedrow, John R||Kaminski, Naftali||Kim, Woo Jin||Lee, Chun Geun||Oh, Yeon-Mok||Elias, Jack A