Modulation of flavanone and furocoumarin levels in grapefruits (Citrus paradisi Macfad.) by production and storage conditions.
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Grapefruits grown under organic or conventional systems were analyzed for 6,7-dihydroxybergamottin (DHB) and flavanones using HPLC, and DPPH activity and ORAC using a micro-plate reader. Grapefruits harvested in November 2008 (E-1) and February 2010 (E-2) were stored at room temperature (RT) and 9 C for four weeks. Higher levels of DHB were observed in conventional grapefruits during the second (4.7 0.2 g/g), third (1.5 0.2 g/g) and fourth (2.5 0.2 g/g) week of storage at room temperature in E2. Among flavonoids analyzed, narirutin (666.7 33.9 g/g), neohesperidin (17.5 1.3 g/g), didymin (75.5 5.6 g/g) and poncirin (130.8 10.4 g/g) levels were significantly higher (P0.05) in organic grapefruits over conventional grapefruits at harvest and storage in E-1. Although DPPH levels were moderately correlated with grapefruit flavanone content, variability in the individual flavanone activity was pronounced, resulting in non-significant differences in antioxidant activity between organic and conventional grapefruits.