Effects of Polyphenolics from Grape (Vitis rotundifolia) and acai (Euterpe oleracea Mart.) on the expresion of microRNAs relevant to inflammation in vascular diseases Conference Paper uri icon


  • The cardioprotective and antiinnflammatory effects of polyphenolics have been demonstrated. It was the objective to investigate the antiinflammatory effects of polyphenolics from grape (Gp) and aai (Ac) in human umbilical vein endothelial cells (HUVEC). Cells were treated with Ac or Gp phenolics (520 mg/L) and inflammation was induced with glucose (Gluc) (25mM) or lipopolysaccharide (LPS) (1ng/mL). The generation of reactive oxygen species (ROS) was determined with the dichlorofluorescein assay. MicroRNAs and mRNAs were quantified by RTPCR. Ac and Gp phenolics (540mg/L) did not inhibit cell proliferation and moreover protected cells against Gluc induced ROS (Ac: 21 57% and Gp: 37 40%). Ac and Gp reversed Glucinduced 7.2fold increased expression of mir146a down to control levels in a dosedependent manner. Additionally, Ac and Gp reduced the mRNA expression of tumor necrosis factor receptor I (TNFRI) (up to 66%) and interleukin (IL8) (up to 68%). Gp and Ac decreased LPSinduced expression of ICAM (up to 4060%) and angiotensin II (AGII) (up to 15%), and correspondingly increased expression of mir126 and mir155 (up to 3.8 and 4.4 fold, respectively) which are known inhibitors of ICAM and AGII, respectively. Overall, the investigated microRNAs may play a key role in the underlying mechanisms of the antiinflammatory effects of Gp and Ac polyphenolics.

published proceedings


author list (cited authors)

  • Noratto, G. D., Angel, G., & Mertens-Talcott, S. U.

citation count

  • 0

complete list of authors

  • Noratto, Giuliana Doris||Angel, Gabriela||Mertens-Talcott, Susanne U

publication date

  • January 2009