Resveratrol and quercetin target microRNA 27a in their anticancer effects in colon cancer cells Conference Paper uri icon


  • The polyphenolics resveratrol and quercetin in combination previously were shown to exert synergistic anticarcinogenic effects. The objective was to investigate anticancer properties and underlying mechanisms of resveratrol and quercetin in combination in colorectal adenocarcinoma cells (HT29). Cell viability was determined after 24, 48, 72 and 96h, and IC50 values were 62, 24, 21 and 14 mg/L (combined concentration of resveratrol and quercetin in 1:1 ratio), respectively. Reactive oxygen species (ROS) increased up to 2.25fold at 530mg/L and mRNA of the cell cycle regulator p53 and the apoptotic enzyme caspase3 were increased by up to 2fold and 2.5fold within a concentration range of 2060 mg/L after 24h, respectively. mRNA of zincfinger protein ZBTB10 a putative suppressor of the oncogenic specificity protein Sp1, was significantly increased by 3.5fold at 30 mg/L, coinciding with a significant decrease in Sp1mRNA levels. miRNA27a, which in previous studies was determined to be a downregulator of ZBTB10, was upregulated by 2.5fold, indicating the involvement of this miRNA in the downregulation of Sp1 protein and potentially the induction of apoptosis. In conclusion, the combination of resveratrol and quercetin was highly effective in the reduction of cell viability in colon cancer cells, employing apoptosis and the inhibition of microRNA27a as underlying mechanism.Grant Funding SourceTexas A&M University

published proceedings


author list (cited authors)

  • Del Follo, A., Noratto, G. D., & Mertens-Talcott, S.

citation count

  • 0

complete list of authors

  • Del Follo, Armando||Noratto, Giuliana D||Mertens-Talcott, Susanne

publication date

  • April 2009