Dietary Supplementation with Raspberry Whole Fruit Modifies the Relative Abundance of Fecal Microbial Communities in Obese Diabetic (db/db) Mice
Conference Paper
Overview
Identity
Additional Document Info
Other
View All
Overview
abstract
We studied if dietary supplementation with red raspberries can modulate the fecal microbiota of db/db mice. Fecal samples from db/db mice fed a raspberrysupplemented diet (n=12) or an isocaloric control diet (n=15) were used for microbial description using 16S rDNA sequencing with a MiSeq instrument (Illumina). Microbial taxonomy assignments were based on a curated goodquality 16S database (GreenGenes). A total of 2.6 million goodquality sequences (average: 272 nucleotide long) were available for taxonomic and diversity analysis. These sequences were assigned to 3,857 different groups of sequences or Operational Taxonomy Units.There were no significant treatment differences in the two more predominant phyla Firmicutes and Bacteroidetes, each comprising of many different species of bacteria. Interestingly, when looking at deeper taxonomic levels within each phylum, raspberry supplementation was associated with higher relative proportions of Lachnospiraceae, a group that has been shown to be depleted during disease processes inside the gut such as inflammatory bowel disease. Raspberrysupplemented obese mice also showed relatively lower abundance of an interesting fiber degrader (family S247). This is important to point out because the combined effects of polyphenolic compounds and fiber in raspberries have not been studied. Changes in Lachnospiraceae, S247 and a few other groups adequately differentiated treatment differences in the whole microbial composition. In conclusion, consumption of raspberry is associated with changes in fecal microbial communities of db/db mice; thus future studies on the potential beneficial effect of raspberries on intestinal health are warranted.Support or Funding InformationNational Raspberry Council
