Tooth Root Formation: An Emerging Signaling Pathway
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abstract
Despite the advances made in identifying the signaling pathways in tooth germ and crown morphogenesis, the precise molecular events leading to root formation are largely unknown. Since dentin forms the bulk of the root, such knowledge is critical for exploring how pulpal cells respond to injury by forming a reparative dentin matrix and for developing predictable therapies for dentin-pulp complex repair. The current belief is that root development is initiated from HERS (Hertwig''s Epithelial Root Sheath), but amelogenesis imperfecta patients exhibit no apparent root defects. Our recent data showed that the target deletion of Bmpr1a in epithelium by keratin 5 Cre induced at E14.5 leads to severe enamel defects but showed no apparent root dentin phenotype, suggesting that molecules originating from mesenchymal cells may play critical roles during root formation. The root dentinogenesis model offers a practical means of studying how odontoblast differentiation is regulated at the molecular level. In this study, we will perform a series of experiments to testthe hypothesis that BMPR1A signaling originating from pulp/odontoblast cells controls root formation via a novel NFIC-OSX-¿-catenin-DSPP signaling pathway. We have proposed three Aims. In specific Aim 1 we will test the hypothesis that OSX is a key downstream molecule of BMPR1A. To test this hypothesis, we will use the 3.2 Col 1ERTM-Cre (induced by injection of Tamoxifen) to determine the degree to which re-expression of Osx1 can rescue the abnormal root phenotype in Bmpr1a cKO. In Specific Aim 2 we will test the hypothesis that OSX controls root dentinogenesis through (i) the inhibition of cell proliferation and (ii) the acceleration of cll differentiation via the inhibition of ?-catenin and upregulation of DSPP. In Specific Aim 3 we willtest the hypothesis that NFIC, an essential transcriptional factor for root dentinogenesis, is a ke downstream molecule of BMP, which controls root dentinogenesis via OSX. To test this hypothesis, we will re-express Nfic in the Bmpr1a cKO osteoblasts to determine the degree to which Nfic can rescue the abnormal root phenotype. We will also re-express Osx in the Nfic KO odontoblasts to determine the degree to which re-expression of Osx can rescue the abnormal root phenotype. Upon completion of this project in an understudied area, we will provide new mechanistic insights into the role of these signaling molecules from mesenchymal cells during root formation. This valuable information will not only challenge the current dogma (induction of root signal is from HERS) but will also be applied to the development of new bioactive restorative therapies aimed at restoring the integrity of the dentin-pulp complex after injury.
