Repair of dentin defects from DSPP knockout mice by PILP mineralization. Academic Article uri icon

abstract

  • Dentinogenesis imperfecta type II (DGI-II) lacks intrafibrillar mineral with severe compromise of dentin mechanical properties. A Dspp knockout (Dspp-/-) mouse, with a phenotype similar to that of human DGI-II, was used to determine if poly-L-aspartic acid [poly(ASP)] in the "polymer-induced liquid-precursor" (PILP) system can restore its mechanical properties. Dentin from six-week old Dspp-/- and wild-type mice was treated with CaP solution containing poly(ASP) for up to 14 days. Elastic modulus and hardness before and after treatment were correlated with mineralization from Micro x-ray computed tomography (Micro-XCT). Transmission electron microscopy (TEM)/Selected area electron diffraction (SAED) were used to compare matrix mineralization and crystallography. Mechanical properties of the Dspp-/- dentin were significantly less than wild-type dentin and recovered significantly (P < 0.05) after PILP-treatment, reaching values comparable to wild-type dentin. Micro-XCT showed mineral recovery similar to wild-type dentin after PILP-treatment. TEM/SAED showed repair of patchy mineralization and complete mineralization of defective dentin. This approach may lead to new strategies for hard tissue repair.

published proceedings

  • J Mater Res

author list (cited authors)

  • Nurrohman, H., Saeki, K., Carneiro, K., Chien, Y. C., Djomehri, S., Ho, S. P., ... Habelitz, S.

citation count

  • 20

complete list of authors

  • Nurrohman, H||Saeki, K||Carneiro, K||Chien, YC||Djomehri, S||Ho, SP||Qin, C||Marshall, SJ||Gower, LB||Marshall, GW||Habelitz, S

publication date

  • January 2016