Flavonol-rich fractions of yaupon holly leaves (Ilex vomitoria, Aquifoliaceae) induce microRNA-146a and have anti-inflammatory and chemopreventive effects in intestinal myofibroblast CCD-18Co cells. Academic Article uri icon

abstract

  • Polyphenolics extracted from yaupon holly (Ilex vomitoria, Aquifoliaceae) (YH) leaves were investigated in human colon cells for their chemopreventive and anti-inflammatory activities. An activity-guided fractionation allowed the selection of YH flavonol-rich fraction due to its preferential inhibition of HT-29 colon cancer viability over the normal CCD-18Co colon cells. Quercetin and kaempferol 3-rutinosides, main components identified in this fraction, protected CCD-18Co cells against reactive oxidative species (ROS) in part due to increased activity of antioxidant enzymes. In addition, up-regulation of microRNA-146a (miR-146a) known as a negative regulator of pro-inflammatory NF-B activation was the underlying molecular mechanism that protected CCD-18Co from inflammation.

published proceedings

  • Fitoterapia

altmetric score

  • 1.75

author list (cited authors)

  • Noratto, G. D., Kim, Y., Talcott, S. T., & Mertens-Talcott, S. U.

citation count

  • 56

complete list of authors

  • Noratto, Giuliana D||Kim, Youngmok||Talcott, Stephen T||Mertens-Talcott, Susanne U

publication date

  • June 2011