Pro-apoptotic activities of polyphenolics from aai (Euterpe oleracea Martius) in human SW-480 colon cancer cells.
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This study aimed to evaluate the cell growth inhibition activity of aai (Euterpe oleracea Mart.) polyphenolic extract against colon cancer HT-29 and SW-480 cells and the nonmalignant CCD-18Co colon fibroblast cells. Results showed that aai polyphenolic extract (5-20 mg/L) inhibited preferentially the growth of SW-480 cells with no toxicity in CCD-18Co cells, and this was accompanied by reduction of H2O2-induced reactive oxygen species (ROS) generation. The mechanisms involved in SW-480 cell growth-inhibition by aai polyphenolic extract included the downregulation of NF-B proinflammatory transcription factor and the nuclear factor-kappa B targets intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Furthermore, prooncogenic specificity proteins (Sp) were downregulated as well as Sp-targets Bcl-2, vascular endothelial growth factor, and survivin. This was accompanied by activation of mitochondrial proapoptotic pathway involving increase of cytochrome c, cleavage of caspase-3, and decrease of PARP-1. Results strongly suggest that aai polyphenolic extract has antiinflammatory and cytotoxic activities in colon cancer cells and can be effective as natural colon cancer chemopreventive agents.
author list (cited authors)
Dias, M., Noratto, G., Martino, H., Arbizu, S., Peluzio, M., Talcott, S., Ramos, A. M., & Mertens-Talcott, S. U.
complete list of authors
Dias, Manoela Maciel dos Santos||Noratto, Giuliana||Martino, Hercia Stampini Duarte||Arbizu, Shirley||Peluzio, Maria do Carmo Gouveia||Talcott, Stephen||Ramos, Afonso Mota||Mertens-Talcott, Susanne U