Pro-apoptotic activities of polyphenolics from aai (Euterpe oleracea Martius) in human SW-480 colon cancer cells. Academic Article uri icon

abstract

  • This study aimed to evaluate the cell growth inhibition activity of aai (Euterpe oleracea Mart.) polyphenolic extract against colon cancer HT-29 and SW-480 cells and the nonmalignant CCD-18Co colon fibroblast cells. Results showed that aai polyphenolic extract (5-20 mg/L) inhibited preferentially the growth of SW-480 cells with no toxicity in CCD-18Co cells, and this was accompanied by reduction of H2O2-induced reactive oxygen species (ROS) generation. The mechanisms involved in SW-480 cell growth-inhibition by aai polyphenolic extract included the downregulation of NF-B proinflammatory transcription factor and the nuclear factor-kappa B targets intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Furthermore, prooncogenic specificity proteins (Sp) were downregulated as well as Sp-targets Bcl-2, vascular endothelial growth factor, and survivin. This was accompanied by activation of mitochondrial proapoptotic pathway involving increase of cytochrome c, cleavage of caspase-3, and decrease of PARP-1. Results strongly suggest that aai polyphenolic extract has antiinflammatory and cytotoxic activities in colon cancer cells and can be effective as natural colon cancer chemopreventive agents.

published proceedings

  • Nutr Cancer

altmetric score

  • 11.75

author list (cited authors)

  • Dias, M., Noratto, G., Martino, H., Arbizu, S., Peluzio, M., Talcott, S., Ramos, A. M., & Mertens-Talcott, S. U.

citation count

  • 26

complete list of authors

  • Dias, Manoela Maciel dos Santos||Noratto, Giuliana||Martino, Hercia Stampini Duarte||Arbizu, Shirley||Peluzio, Maria do Carmo Gouveia||Talcott, Stephen||Ramos, Afonso Mota||Mertens-Talcott, Susanne U

publication date

  • November 2014