Anti-lipidaemic and anti-inflammatory effect of aai ( Euterpe oleracea Martius) polyphenols on 3T3-L1 adipocytes
- Additional Document Info
- View All
2016 Elsevier Ltd. The anti-lipidaemic and anti-inflammatory effects of aai polyphenols in 3T3-L1 mouse adipocytes were investigated. Pre-adipocytes were differentiated with and without aai-polyphenols at concentrations of 2.5, 5 and 10 g gallic acid equivalents (GAE)/mL. Results showed that aai polyphenols reduce the accumulation of intracellular lipids in differentiated adipocytes in a dose-dependent manner and downregulated PPAR2. The gene-expression of adipogenic transcription factors C/ebp, C/ebp, Klf5 and Srebp1c was decreased. This was accompanied by a reduction of adipogenic genes, including aP2, LPL, FATP1 and FAS, leptin and total PAI and an increase of adiponectin. Additionally, aai polyphenols protected cells against the production of ROS and decreased the expression of mRNA and protein levels of pro-inflammatory cytokines when 3T3-L1 cells were challenged with TNF-. Thus, these results indicate that aai polyphenols may be useful in the prevention of adipogenesis, oxidative stress and inflammation.
Journal of Functional Foods
author list (cited authors)
Martino, H., Dias, M., Noratto, G., Talcott, S., & Mertens-Talcott, S. U.
complete list of authors
Martino, Hércia Stampini Duarte||Dias, Manoela Maciel dos Santos||Noratto, Giuliana||Talcott, Stephen||Mertens-Talcott, Susanne U