Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor. Academic Article

abstract

• We have sequenced a cloned segment of E. coli chromosomal DNA that includes the heat shock regulatory gene htpR. This segment contains an 852 nucleotide open reading frame bounded by transcriptional and translational signals. Both in vivo and in vitro the cloned segment produces a single protein that migrates in gels with the cellular protein (F33.4) implicated as the htpR product. Properties of a cloned fragment of the coding sequence truncated at the promoter-distal end are consistent with this assignment. The htpR gene product appears homologous to the sigma factor of RNA polymerase, and the two proteins are predicted to have similar secondary structure. In addition, two regions of the predicted htpR product resemble protein-DNA contact points conserved in known DNA-binding proteins.

authors

• Erickson, James

published proceedings

• Cell

author list (cited authors)

• Landick, R., Vaughn, V., Lau, E. T., VanBogelen, R. A., Erickson, J. W., & Neidhardt, F. C.

citation count

• 180

complete list of authors

• Landick, R||Vaughn, V||Lau, ET||VanBogelen, RA||Erickson, JW||Neidhardt, FC

publication date

• August 1984

publisher

• Elsevier  Publisher

published in

• Cell  Journal

keywords

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA Restriction Enzymes
• Escherichia Coli
• Genes
• Genes, Bacterial
• Genes, Regulator
• Heat-Shock Proteins
• Transcription Factors

PubMed Central ID

• 6088062

Digital Object Identifier (DOI)

• 10.1016/0092-8674(84)90538-5

start page

• 175

end page

• 182

volume

• 38

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2F0092-8674%2884%2990538-5