Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications. Academic Article

abstract

• 2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency.

authors

• Liu, Wenshe
• Russell, David

published proceedings

• Bioorg Med Chem Lett

author list (cited authors)

• Huang, Y., Wan, W., Russell, W. K., Pai, P., Wang, Z., Russell, D. H., & Liu, W.

citation count

• 50

complete list of authors

• Huang, Ying||Wan, Wei||Russell, William K||Pai, Pei-Jing||Wang, Zhiyong||Russell, David H||Liu, Wenshe

publication date

• February 2010

publisher

• Elsevier  Publisher

published in

• Bioorganic and Medicinal Chemistry Letters  Journal

keywords

• Amino Acid Sequence
• Amino Acids
• Binding Sites
• Escherichia Coli Proteins
• Fatty Acids
• Keto Acids
• Molecular Sequence Data
• Protein Engineering

PubMed Central ID

• 20074948

Digital Object Identifier (DOI)

• 10.1016/j.bmcl.2009.12.077

start page

• 878

end page

• 880

volume

• 20

issue

• 3

URL

• http://dx.doi.org/10.1016/j.bmcl.2009.12.077