Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications. Academic Article uri icon


  • 2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency.

published proceedings

  • Bioorg Med Chem Lett

author list (cited authors)

  • Huang, Y., Wan, W., Russell, W. K., Pai, P., Wang, Z., Russell, D. H., & Liu, W.

citation count

  • 50

complete list of authors

  • Huang, Ying||Wan, Wei||Russell, William K||Pai, Pei-Jing||Wang, Zhiyong||Russell, David H||Liu, Wenshe

publication date

  • December 2009