Fan, Lin (2003-05). The Tsr chemoreceptor/CheW/CheA ternary complex as an allosteric enzyme. Master's Thesis. Thesis uri icon

abstract

  • The transmembrane serine receptor Tsr associates with a coupling protein, CheW, and a histidine kinase, CheA, to form a ternary complex that regulates the activity of CheA. CheA activity is inhibited by binding of L-serine to Tsr. This work aims to characterize the ligand-binding properties of Tsr and the inhibitory effect of L-serine on CheA activity. The periplasmic domain of Tsr (pTsr) was purified and characterized. Analytical gel filtration and analytical ultracentrifugation indicated that binding of Lserine promotes dimerization. The binding stoichiometry and dissociation constant for binding of L-serine to pTsr were determined by fluorescence spectroscopy. As protein concentration decreased, the dissociation constant increased. A working model was proposed to account for the interactions between L-serine and pTsr. The activity of CheA in a ternary complex with full-length Tsr and CheW was analyzed by measuring the production of [32P]-phospho-CheY. (Phospho-CheY is the product of CheA catalysis.) The results revealed that binding of L-serine decreased CheA activity without changing its affinity for ATP. These findings suggest that the allosteric effect of L-serine on CheA activity might occur through V-type inhibition. Optimization of an alternative, continuous, non-radioactive assay for CheA is underway.
  • The transmembrane serine receptor Tsr associates with a coupling protein, CheW,
    and a histidine kinase, CheA, to form a ternary complex that regulates the activity of
    CheA. CheA activity is inhibited by binding of L-serine to Tsr. This work aims to
    characterize the ligand-binding properties of Tsr and the inhibitory effect of L-serine on
    CheA activity. The periplasmic domain of Tsr (pTsr) was purified and characterized.
    Analytical gel filtration and analytical ultracentrifugation indicated that binding of Lserine
    promotes dimerization. The binding stoichiometry and dissociation constant for
    binding of L-serine to pTsr were determined by fluorescence spectroscopy. As protein
    concentration decreased, the dissociation constant increased. A working model was
    proposed to account for the interactions between L-serine and pTsr. The activity of
    CheA in a ternary complex with full-length Tsr and CheW was analyzed by measuring
    the production of [32P]-phospho-CheY. (Phospho-CheY is the product of CheA catalysis.)
    The results revealed that binding of L-serine decreased CheA activity without changing
    its affinity for ATP. These findings suggest that the allosteric effect of L-serine on CheA activity might occur through V-type inhibition. Optimization of an alternative,
    continuous, non-radioactive assay for CheA is underway.

publication date

  • May 2003