R01 HL074236 Chemokines and Immune Cells in Hind Limb Ischemia
Grant
Overview
Affiliation
Other
View All
Overview
abstract
This proposal tests the overall hypothesis that the MCP-1/CCR2 axis is critical for wound healing after the induction of hind limb ischemia. Chimeras will be used to determine the effect of the hematopoietic versus nonhematopoietic compartments in this process. This grant was competitively renewed, studying the effect of macrophages on angiogenesis and tissue regeneration after ischemic injury. The following 3 specific aims tested the overall hypothesis that the recruitment and activation of bone marrow-derived cells, especially monocytes/macrophages, are essential for angiogenesis, a critical component in skeletal muscle regeneration after injury. 1) Determine the contribution of CCR2 expression in bone marrow-derived vs non-bone marrow-derived cells on angiogenesis in skeletal muscle after injury, 2) Determine the ex vivo and in vivo influence of the MCP- 1/CCR2 axis on angiogenesis and 3) Define the effects of selective and complete monocyte/macrophage ablation prior to muscle injury and sustained throughout the time course of tissue repair on inflammation and angiogenesis after injury.
This Award also was awarded two postdoctoral diversity supplements to support the training of Drs. Ochoa and Wells.
