A rapid assay for 14C-labeled hydroxylysine in collagen and related materials.
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A rapid technique for assaying 14C-labeled hydroxylysine in biological materials is reported. The assay depends on the periodate oxidation of 14C-hydroxylysine to give 14C-formaldehyde. The 14C-formaldehyde is reacted with dimedon and the resulting 14C-formaldemethone complex is extracted into toluene. The method should be applicable to studying the synthesis and metabolism of hydroxylysine in collagen and in related glycoproteins of tissues such as basement membranes. The method distinguishes between glycosylated and nonglycosylated 14C-hydroxylysine, and therefore it is applicable to studying the glycosylation of hydroxylysine in collagen and similar proteins. 1969.
author list (cited authors)
Blumenkrantz, N., & Prockop, D. J.
complete list of authors
Blumenkrantz, N||Prockop, DJ