A rapid assay for 14C-labeled hydroxylysine in collagen and related materials. Academic Article

abstract

• A rapid technique for assaying 14C-labeled hydroxylysine in biological materials is reported. The assay depends on the periodate oxidation of 14C-hydroxylysine to give 14C-formaldehyde. The 14C-formaldehyde is reacted with dimedon and the resulting 14C-formaldemethone complex is extracted into toluene. The method should be applicable to studying the synthesis and metabolism of hydroxylysine in collagen and in related glycoproteins of tissues such as basement membranes. The method distinguishes between glycosylated and nonglycosylated 14C-hydroxylysine, and therefore it is applicable to studying the glycosylation of hydroxylysine in collagen and similar proteins. 1969.

authors

• Prockop, Darwin

published proceedings

• Anal Biochem

author list (cited authors)

• Blumenkrantz, N., & Prockop, D. J.

citation count

• 38

complete list of authors

• Blumenkrantz, N||Prockop, DJ

publication date

• January 1969

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Amino Acids
• Animals
• Basement Membrane
• Carbon Isotopes
• Chick Embryo
• Chromatography
• Collagen
• Glycoproteins
• Indicators And Reagents
• Lysine
• Methods
• Microbial Collagenase
• Periodic Acid

Digital Object Identifier (DOI)

• 10.1016/0003-2697(69)90130-4

start page

• 377

end page

• 385

volume

• 30

issue

• 3

URL

• http://dx.doi.org/10.1016/0003-2697(69)90130-4