MYELIN SYNTHESIS IN PERIPHERAL NERVE IN VITRO: SULPHATIDE INCORPORATION REQUIRES A TRANSPORT LIPOPROTEIN1,2,3
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Abstract— Sciatic nerves from 18‐day‐old chick embryos incorporated 35SO4 into myelin sulphatide in vitro. Sulphatide in a microsomal subfraction of the nerve was rapidly labelled with 35SO4, and a lipoprotein fraction in the nerve served to transfer the [35S]sulphatide from the microsomal subfraction to myelin. Puromycin and cycloheximide inhibited the incorporation of [35S]sulphatide into myelin after a lag period of about 2 h. These agents did not alter the rate of appearance of [35S]sulphatide in the microsomal subfraction, and did not diminish the capacity of myelin to take up [35S]sulphatide from the lipoprotein fraction; instead, they appeared to interfere with the incorporation of [35S]sulphatide into myelin by decreasing the available pool of the transport lipoprotein. Partial characterization of the [35S]labelled lipoprotein fraction indicated that it had a density of 1.06–1.08. The lipoprotein was highly aggregated, but, after incubation with SDS and mercaptoethanol, it was dissociated into sulphatide‐containing micelles and proteins. Copyright © 1972, Wiley Blackwell. All rights reserved
author list (cited authors)
Pleasure, D. E., & Prockop, D. J.