Myelin synthesis in peripheral nerve in vitro: sulphatide incorporation requires a transport lipoprotein.
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Abstract Sciatic nerves from 18dayold chick embryos incorporated 35SO4 into myelin sulphatide in vitro. Sulphatide in a microsomal subfraction of the nerve was rapidly labelled with 35SO4, and a lipoprotein fraction in the nerve served to transfer the [35S]sulphatide from the microsomal subfraction to myelin. Puromycin and cycloheximide inhibited the incorporation of [35S]sulphatide into myelin after a lag period of about 2 h. These agents did not alter the rate of appearance of [35S]sulphatide in the microsomal subfraction, and did not diminish the capacity of myelin to take up [35S]sulphatide from the lipoprotein fraction; instead, they appeared to interfere with the incorporation of [35S]sulphatide into myelin by decreasing the available pool of the transport lipoprotein. Partial characterization of the [35S]labelled lipoprotein fraction indicated that it had a density of 1.061.08. The lipoprotein was highly aggregated, but, after incubation with SDS and mercaptoethanol, it was dissociated into sulphatidecontaining micelles and proteins. Copyright 1972, Wiley Blackwell. All rights reserved
author list (cited authors)
Pleasure, D. E., & Prockop, D. J.
complete list of authors
Pleasure, DE||Prockop, DJ