Dissociation of the synthesis of sulphated mucopolysaccharides and the synthesis of collagen in embryonic cartilage.
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The possibility of an interrelationship between synthesis of collagen and the synthesis of sulphated mucopolysaccharides in embryonic cartilage was examined by selectivity inhibiting these synthetic pathways. When the synthesis of collagen was inhibited with ,-dipyridyl, the incorporation of d-[i-14C]glucosamine and [35S]-sulphate into non-dialyzable fractions continued. Inhibition of mucopolysaccharide synthesis with 6-diazo-5-oxonorleucine, an analogue of gluthamine, did not affect the biosynthesis of collagen. Autoradiographic studies showed that the synthesis of sulphated mucopolysaccharides could be inhibited without affecting the extrusion of collgen into the extracellular matrix, suggesting that the formation of a complex between collagen and mucopolysaccharides is not a necessary step for the incorporation of collagen into extracellular matrix. Observations reported here indicate that the synthetic processes leading to the formation of collagen and mucopolysaccharides may be dissociated, and they suggest that there is no essential interrelationship between the two pathways. 1966.
author list (cited authors)
Bhatnagar, R. S., & Prockop, D. J.
complete list of authors
Bhatnagar, RS||Prockop, DJ