Robotic automation of dideoxyribonucleotide sequencing reactions. Academic Article

abstract

• We developed a robot to carry out standard Sanger dideoxyribonucleotide sequencing reactions efficiently and with minimal human intervention. A commercial robot was adapted to our design and specifications, and we programmed it to perform up to 240 sequencing reactions in a single unattended run of 7 h. The robot configuration can be easily altered to allow 480 reactions to be performed in an unattended run of 14 h. The special features of our robot include cooled reagent reservoirs and cooled chambers for storage of DNA templates and completed reactions as well as reproducible aspiration of small volumes by using a sensing algorithm. The robot has successfully performed over 3500 DNA reactions in about 30 separate runs in our DNA core facility.

authors

• Prockop, Darwin

published proceedings

• Biotechniques

author list (cited authors)

• Earley, J. J., Kuivaniemi, H., Prockop, D. J., & Tromp, G.

citation count

• 8

complete list of authors

• Earley, JJ||Kuivaniemi, H||Prockop, DJ||Tromp, G

publication date

• July 1994

published in

• BioTechniques: the journal of laboratory technology for bioresearch  Journal

keywords

• DNA-Directed DNA Polymerase
• Robotics
• Sequence Analysis, DNA

PubMed Central ID

• 7946299

start page

• 156

end page

• 165

volume

• 17

issue

• 1