Isolation of a highly clonogenic and multipotential subfraction of adult stem cells from bone marrow stroma. Academic Article

abstract

• Attempts have been made to develop cell and gene therapies using the adult stem cells from bone marrow referred to as mesenchymal stem cells or marrow stromal cells (MSCs). However, the results have been variable in part because there are no standardized protocols for preparing and characterizing MSCs. In the experiments presented here, we developed a standardized assay by light scattering to measure the content of rapidly self-renewing cells (RS cells) in preparations of MSCs. The assay quickly identifies preparations of MSCs that replicate rapidly in subsequent culture. In addition, the standardized assay enabled us to isolate RS cells that were up to 90% clonogenic and that generated single cell-derived colonies that differentiated into either mineralizing cells or adipocytes with appropriate additions to the medium.

authors

• Prockop, Darwin

published proceedings

• Stem Cells

altmetric score

• 1

author list (cited authors)

• Smith, J. R., Pochampally, R., Perry, A., Hsu, S., & Prockop, D. J.

citation count

• 186

complete list of authors

• Smith, Jason R||Pochampally, Radhika||Perry, Anthony||Hsu, Shu-Ching||Prockop, Darwin J

publication date

• September 2004

publisher

• Oxford University Press (OUP)  Publisher

published in

• Stem Cells  Journal

keywords

• Adipocytes
• Bone Marrow Cells
• Bone Marrow Transplantation
• Cell Culture Techniques
• Cell Differentiation
• Cell Lineage
• Cell Proliferation
• Cell Separation
• Cells, Cultured
• Clone Cells
• Colony-Forming Units Assay
• Culture Media
• Gene Expression Regulation
• Humans
• Multipotent Stem Cells
• Osteoblasts
• RNA, Messenger
• Stem Cell Transplantation
• Stromal Cells

PubMed Central ID

• 15342946

Digital Object Identifier (DOI)

• 10.1634/stemcells.22-5-823

start page

• 823

end page

• 831

volume

• 22

issue

• 5

URL

• http://dx.doi.org/10.1634/stemcells.22-5-823