Microfluidic microbial fuel cell array for multiplexed long-term parallel analysis of microbial activities
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abstract
A microfluidic microbial fuel cell array as a high-throughput screening platform for long-term studies of electricity-generating microbes has been developed. The device has 24 microfluidically-controlled miniaturized MFCs integrated on a single chip. Both periodic and continuous anolyte/catholyte replenishments can be performed, enabling long-term multiplexed MFC studies. Conversion efficiencies of lactate, acetate, xylose and TSB to electricity by Shewanella oneidensis MR-1 and an environmental isolate Shewanella sp. Hac353 in MFCs were characterized. Using this microfluidic MFC array, MFC efficiencies of different microbe-substrate combinations could be effectively analyzed, demonstrating the utility of the developed MFC array as a high-throughput screening platform for microbial metabolism studies. Copyright (2011) by the Chemical and Biological Microsystems Society.
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15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011