Isolation and characterization of mouse monoclonal antibodies against a human vascular endothelial cell-specific antigen.

A murine hybridoma clone was isolated secreting IgG-antibodies specific for human vascular endothelial cells. The antibody recognized a plasma membrane antigen (designated HECMA-112) of an apparent molecular weight of 112,000. Indirect immunofluorescence with cultured endothelial cells showed a staining pattern clearly distinct from previously described endothelial cell-specific antigens. The antibody bound to untreated as well as ethanol-fixed, confluent human umbilical vein endothelial cells (HUVEC) but not to other human primary cells (corneal endothelial cells, fibroblasts, keratocytes, lymphocytes, thrombocytes, or erythrocytes) or endothelial cells isolated from bovine or porcine aorta. HECMA-112 can be regarded as an additional marker for human vascular endothelial cells in vivo and in vitro.

Isolation and characterization of mouse monoclonal antibodies against a human vascular endothelial cell-specific antigen. Academic Article