n-3 Polyunsaturated Fatty Acids Suppress T-Cell Mitochondrial Translocation to the Immunological Synapse Conference Paper uri icon


  • T helper (Th) cell activation is necessary for the adaptive immune response. Formation of the immunological synapse (IS) between Th cells and antigenpresenting cells is the first step in Th cell activation. In vitro studies indicate that formation of the IS induces cytoskeletondependent mitochondrial redistribution to the immediate vicinity of the IS. This redistribution of mitochondria to the IS in T cells is necessary to maintain Ca2+ influx across the plasma membrane and Ca2+dependent Th cell activation. In an earlier study, we demonstrated that n3 polyunsaturated fatty acids (PUFA) suppress the localization and activation of signaling proteins at the IS. Therefore, we hypothesized that n3 PUFA suppress CD4+ Tcell mitochondrial translocation during the early stages of IS formation. CD4+ cells derived from fat1 mice, a transgenic model that synthesizes n3 PUFA from n6 PUFA, were cocultured with antiCD3expressing hybridoma cells (1452C11) for 15 min at 37C, and mitochondrial relocation to the IS was assessed by confocal microscopy. Tcells from fat1 mice significantly reduced the percentage of cells with mitochondria which translocated to the IS; fat1 (34%) vs. wild type control (85%); P=0.008. These results demonstrate that n3 PUFA limit mitochondrial translocation to the IS, a critical early step for CD4+ Tcell activation.Supported in part by NIH grant DK07107, CA59034 and P30ES09106Grant Funding SourceNIH DK71707 and CA59034

published proceedings


author list (cited authors)

  • Yog, R., Barhoumi, R., McMurray, D. N., & Chapkin, R. S.

citation count

  • 0

complete list of authors

  • Yog, Rajeshwari||Barhoumi, Rola||McMurray, David N||Chapkin, Robert S

publication date

  • January 2009