Microlymphatic flow using fast video microscopy
Additional Document Info
Despite advances in the measurement of lymphatic flow, little is known about the actual velocities of flow in microlymphatic (100 um diameter) vessels. In this paper, video microscopy and particle tracking methods were adapted and integrated with an ultra high-speed imaging camera to obtain measurements of high-speed lymph velocities that previous systems were incapable of measuring. In this study, a mesenteric microlymphatic vessel in a loop of the small intestine of a male Sprague-Dawley rat was exteriorized and imaged at a rate of 500 frames per second (fps) for several contraction sequences. Lymph velocity was shown to fluctuate cyclically with the vessel wall contractions and ranged from -1 to 4 mm/sec through a ten second sequence.
name of conference
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII